Characterisation of endogenous i-motif and G-quadruplex DNA structures in human chromatin and their relationship with cancer-associated genomic landscapes

Kelly Irving

Research output: ThesisDoctoral Thesis

116 Downloads (Pure)

Abstract

This thesis focused on establishing a technique to map genome-wide physiological iM formation sites in a chromatin context. Through the development of iM ChIP-seq, iM structures were found to be prevalent genomic features enriched within regulatory regions of the human genome, including active gene promoters. Promoter iMs correlate with higher transcription levels compared to genes without a promoter iM. The development of this technique allowed for the parallel mapping of endogenous iM and G4 structures, allowing for the distinction between functional features associated with the independent and overlapping structures.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Smith, Nicole, Supervisor
  • Evans, Cameron, Supervisor
  • Blancafort, Pilar, Supervisor
Thesis sponsors
Award date14 Nov 2023
DOIs
Publication statusUnpublished - 2023

Fingerprint

Dive into the research topics of 'Characterisation of endogenous i-motif and G-quadruplex DNA structures in human chromatin and their relationship with cancer-associated genomic landscapes'. Together they form a unique fingerprint.

Cite this