Characterisation of Cyclophilin Proteins from Burkholderia pseudomallei and Their Role in Pathogenesis

Nicole Bzdyl

doi:10.26182/mhkc-s862

Characterisation of Cyclophilin Proteins from Burkholderia pseudomallei and Their Role in Pathogenesis

Nicole Bzdyl

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Northern Australia. This study identified two enzymatically active cyclophilin proteins, PpiA and PpiB, as well as characterising their role in pathogenesis. PpiA plays a role in environmental survival and metabolism of B. pseudomallei. PpiB is essential for B. pseudomallei virulence, with deletion causing widespread disruption of the proteome resulting in a lack of cell-to-cell spread in vitro and an inability to cause disease in vivo. PpiB presents as a promising target for the development of anti-virulence compounds against Burkholderia pseudomallei.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Kahler, Charlene, Supervisor Sarkar-Tyson, Mitali, Supervisor Inglis, Tim, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	1 Nov 2021
DOIs	https://doi.org/10.26182/mhkc-s862
Publication status	Unpublished - 2021

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10.26182/mhkc-s862

THESIS - DOCTOR OF PHILOSOPHY - BZDYL, Nicole Malgorzata - 2021
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Cite this

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title = "Characterisation of Cyclophilin Proteins from Burkholderia pseudomallei and Their Role in Pathogenesis",

abstract = "Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Northern Australia. This study identified two enzymatically active cyclophilin proteins, PpiA and PpiB, as well as characterising their role in pathogenesis. PpiA plays a role in environmental survival and metabolism of B. pseudomallei. PpiB is essential for B. pseudomallei virulence, with deletion causing widespread disruption of the proteome resulting in a lack of cell-to-cell spread in vitro and an inability to cause disease in vivo. PpiB presents as a promising target for the development of anti-virulence compounds against Burkholderia pseudomallei.",

keywords = "Microbiology, virulence, Pathogenesis, Immunophilins, cyclophilins, Melioidosis, Molecular biology",

author = "Nicole Bzdyl",

year = "2021",

doi = "10.26182/mhkc-s862",

language = "English",

school = "The University of Western Australia",

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AU - Bzdyl, Nicole

PY - 2021

Y1 - 2021

N2 - Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Northern Australia. This study identified two enzymatically active cyclophilin proteins, PpiA and PpiB, as well as characterising their role in pathogenesis. PpiA plays a role in environmental survival and metabolism of B. pseudomallei. PpiB is essential for B. pseudomallei virulence, with deletion causing widespread disruption of the proteome resulting in a lack of cell-to-cell spread in vitro and an inability to cause disease in vivo. PpiB presents as a promising target for the development of anti-virulence compounds against Burkholderia pseudomallei.

AB - Burkholderia pseudomallei is the causative agent of melioidosis, a disease endemic in Northern Australia. This study identified two enzymatically active cyclophilin proteins, PpiA and PpiB, as well as characterising their role in pathogenesis. PpiA plays a role in environmental survival and metabolism of B. pseudomallei. PpiB is essential for B. pseudomallei virulence, with deletion causing widespread disruption of the proteome resulting in a lack of cell-to-cell spread in vitro and an inability to cause disease in vivo. PpiB presents as a promising target for the development of anti-virulence compounds against Burkholderia pseudomallei.

KW - Microbiology

KW - virulence

KW - Pathogenesis

KW - Immunophilins

KW - cyclophilins

KW - Melioidosis

KW - Molecular biology

U2 - 10.26182/mhkc-s862

DO - 10.26182/mhkc-s862

M3 - Doctoral Thesis

ER -

Characterisation of Cyclophilin Proteins from Burkholderia pseudomallei and Their Role in Pathogenesis

Abstract

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