Changing Pax6 expression correlates with axon outgrowth and restoration of topography during optic nerve regeneration

Jennifer Rodger, C. King, Sherralee Lukehurst, P.B. Chen, Sarah Dunlop, Lyn Beazley, Melanie Ziman

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Pax6, a member of the highly conserved developmental Pax gene family, plays a crucial role in early eye development and continues to be expressed in adult retinal ganglion cells (RGCs). Here we have used Western blots and immunohistochemistry to investigate the expression of Pax6 in the formation and refinement of topographic projections during optic nerve regeneration in zebrafish and lizard. In zebrafish with natural (12-h light/dark cycle) illumination, Pax6 expression in RGCs was decreased during axon outgrowth and increased during the restoration of the retinotectal map. Rearing fish in stroboscopic illumination to prevent retinotopic refinement resulted in a prolonged decrease in Pax6 levels; return to natural light condition's resulted in map refinement and restoration of normal Pax6 levels. In lizard, RGC axons spontaneously regenerate but remain in a persistent state of regrowth and do not restore topography; visual training during regeneration, however, allows a stabilization of connections and return of topography. Pax6 was persistently decreased in untrained animals but remained increased in trained ones. In both species, changes in expression were not due to cell division or cell death. The results suggest that decreased Pax6 expression is permissive for axon regeneration and extensive searching, while higher levels of Pax6 are associated with restoration of topography. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1043-1054
Issue number4
Publication statusPublished - 2006


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