TY - JOUR
T1 - Challenges for standardization of Clostridium difficile typing methods
AU - Huber, C.A.
AU - Foster, Niki
AU - Riley, Thomas
AU - Paterson, D.L.
PY - 2013
Y1 - 2013
N2 - Typing of Clostridium difficile facilitates understanding of the epidemiology of the infection. Some evaluations have shown that certain strain types (for example, ribotype 027) are more virulent than others and are associated with worse clinical outcomes. Although restriction endonuclease analysis (REA) and pulsed-field gel electrophoresis have been widely used in the past, PCR ribotyping is the current method of choice for typing of C. difficile. However, global standardization of ribotyping results is urgently needed. Whole-genome sequencing of C. difficile has the potential to provide even greater epidemiologic information than ribotyping. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
AB - Typing of Clostridium difficile facilitates understanding of the epidemiology of the infection. Some evaluations have shown that certain strain types (for example, ribotype 027) are more virulent than others and are associated with worse clinical outcomes. Although restriction endonuclease analysis (REA) and pulsed-field gel electrophoresis have been widely used in the past, PCR ribotyping is the current method of choice for typing of C. difficile. However, global standardization of ribotyping results is urgently needed. Whole-genome sequencing of C. difficile has the potential to provide even greater epidemiologic information than ribotyping. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
UR - https://www.scopus.com/pages/publications/84882778202
U2 - 10.1128/JCM.00143-13
DO - 10.1128/JCM.00143-13
M3 - Article
SN - 0095-1137
VL - 51
SP - 2810
EP - 2814
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 9
ER -