Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Tim Inglis, M. Aravena-Roman, S. Ching, Kevin Croft, V. Wuthiekanun, Brian Mee

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Abstract

Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.
Original languageEnglish
Pages (from-to)4812-4814
JournalJournal of Clinical Microbiology
Volume41
Issue number10
DOIs
Publication statusPublished - 2003

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