Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Tim Inglis; M. Aravena-Roman; S. Ching; Kevin Croft; V. Wuthiekanun; Brian Mee

doi:10.1128/JCM.41.10.4812-4814.2003

Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Tim Inglis, M. Aravena-Roman, S. Ching, Kevin Croft, V. Wuthiekanun, Brian Mee

Pathology & Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

Original language	English
Pages (from-to)	4812-4814
Journal	Journal of Clinical Microbiology
Volume	41
Issue number	10
DOIs	https://doi.org/10.1128/JCM.41.10.4812-4814.2003
Publication status	Published - 2003

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title = "Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis",

abstract = "Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.",

author = "Tim Inglis and M. Aravena-Roman and S. Ching and Kevin Croft and V. Wuthiekanun and Brian Mee",

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language = "English",

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T1 - Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

AU - Inglis, Tim

AU - Aravena-Roman, M.

AU - Ching, S.

AU - Croft, Kevin

AU - Wuthiekanun, V.

AU - Mee, Brian

PY - 2003

Y1 - 2003

N2 - Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

AB - Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

U2 - 10.1128/JCM.41.10.4812-4814.2003

DO - 10.1128/JCM.41.10.4812-4814.2003

M3 - Article

SN - 0095-1137

VL - 41

SP - 4812

EP - 4814

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 10

ER -

Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Abstract

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