Cell Type of Origin Dictates the Route to Pluripotency

Christian M Nefzger, Fernando J Rossello, Joseph Chen, Xiaodong Liu, Anja S Knaupp, Jaber Firas, Jacob M Paynter, Jahnvi Pflueger, Sam Buckberry, Sue Mei Lim, Brenda Williams, Sara Alaei, Keshav Faye-Chauhan, Enrico Petretto, Susan K Nilsson, Ryan Lister, Mirana Ramialison, David R Powell, Owen J L Rackham, Jose M Polo

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblasts as a universal model for the study of the reprogramming process and provide crucial insights about iPSC generation from alternative cell sources.

Original languageEnglish
Pages (from-to)2649-2660
Number of pages12
JournalCell Reports
Volume21
Issue number10
DOIs
Publication statusPublished - 5 Dec 2017

Fingerprint

Dive into the research topics of 'Cell Type of Origin Dictates the Route to Pluripotency'. Together they form a unique fingerprint.

Cite this