Case study: Applying rapid flow cytometry analysis to CAPD effluent

Kieran T. Mulroney, Jarrad M. Hall, Amanda L. McGuire, Tim J.J. Inglis, Aron Chakera

Research output: Contribution to journalArticle

Abstract

Peritoneal dialysis (PD) peritonitis cases require rapid clinical interventions to ensure the best possible patient outcomes. Culture-dependent microbiology tools are slow and cannot provide clinicians with evidence to guide antimicrobial prescription practices in an appropriate time frame. Genotypic methods have met with limited success for analyzing continuous ambulatory PD effluent, with most centers still relying on culture-dependent microbiology. We present a case study in which we apply flow cytometry techniques to antibiotic-compromised effluent. We demonstrate, with supporting evidence, direct enumeration of bacterial and human immune cells, with results reported within 2 hours of receiving the clinical specimen.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalPeritoneal Dialysis International
Volume38
Issue number5
DOIs
Publication statusPublished - 1 Sep 2018

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