Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria

Elizabeth Ficko-Blean, Aurélie Préchoux, François Thomas, Tatiana Rochat, Robert Larocque, Yongtao Zhu, Mark Stam, Sabine Génicot, Murielle Jam, Alexandra Calteau, Benjamin Viart, David Ropartz, David Pérez-Pascual, Gaëlle Correc, Maria Matard-Mann, Keith A. Stubbs, Hélène Rogniaux, Alexandra Jeudy, Tristan Barbeyron, Claudine Médigue & 5 others Mirjam Czjzek, David Vallenet, Mark J. McBride, Eric Duchaud, Gurvan Michel

Research output: Contribution to journalArticle

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Abstract

Macroalgae contribute substantially to primary production in coastal ecosystems. Their biomass, mainly consisting of polysaccharides, is cycled into the environment by marine heterotrophic bacteria using largely uncharacterized mechanisms. Here we describe the complete catabolic pathway for carrageenans, major cell wall polysaccharides of red macroalgae, in the marine heterotrophic bacterium Zobellia galactanivorans. Carrageenan catabolism relies on a multifaceted carrageenan-induced regulon, including a non-canonical polysaccharide utilization locus (PUL) and genes distal to the PUL, including a susCD-like pair. The carrageenan utilization system is well conserved in marine Bacteroidetes but modified in other phyla of marine heterotrophic bacteria. The core system is completed by additional functions that might be assumed by non-orthologous genes in different species. This complex genetic structure may be the result of multiple evolutionary events including gene duplications and horizontal gene transfers. These results allow for an extension on the definition of bacterial PUL-mediated polysaccharide digestion.

LanguageEnglish
Article number1685
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - 1 Dec 2017

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catabolism
Regulon
Carrageenan
polysaccharides
bacteria
Polysaccharides
Bacteria
genes
loci
Seaweed
Genes
Bacterial Polysaccharides
Bacteroidetes
Gene transfer
Horizontal Gene Transfer
Gene Duplication
Genetic Structures
Biomass
Ecosystems
Cell Wall

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Ficko-Blean, E., Préchoux, A., Thomas, F., Rochat, T., Larocque, R., Zhu, Y., ... Michel, G. (2017). Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria. Nature Communications, 8(1), [1685]. DOI: 10.1038/s41467-017-01832-6
Ficko-Blean, Elizabeth ; Préchoux, Aurélie ; Thomas, François ; Rochat, Tatiana ; Larocque, Robert ; Zhu, Yongtao ; Stam, Mark ; Génicot, Sabine ; Jam, Murielle ; Calteau, Alexandra ; Viart, Benjamin ; Ropartz, David ; Pérez-Pascual, David ; Correc, Gaëlle ; Matard-Mann, Maria ; Stubbs, Keith A. ; Rogniaux, Hélène ; Jeudy, Alexandra ; Barbeyron, Tristan ; Médigue, Claudine ; Czjzek, Mirjam ; Vallenet, David ; McBride, Mark J. ; Duchaud, Eric ; Michel, Gurvan. / Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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Ficko-Blean, E, Préchoux, A, Thomas, F, Rochat, T, Larocque, R, Zhu, Y, Stam, M, Génicot, S, Jam, M, Calteau, A, Viart, B, Ropartz, D, Pérez-Pascual, D, Correc, G, Matard-Mann, M, Stubbs, KA, Rogniaux, H, Jeudy, A, Barbeyron, T, Médigue, C, Czjzek, M, Vallenet, D, McBride, MJ, Duchaud, E & Michel, G 2017, 'Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria' Nature Communications, vol. 8, no. 1, 1685. DOI: 10.1038/s41467-017-01832-6

Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria. / Ficko-Blean, Elizabeth; Préchoux, Aurélie; Thomas, François; Rochat, Tatiana; Larocque, Robert; Zhu, Yongtao; Stam, Mark; Génicot, Sabine; Jam, Murielle; Calteau, Alexandra; Viart, Benjamin; Ropartz, David; Pérez-Pascual, David; Correc, Gaëlle; Matard-Mann, Maria; Stubbs, Keith A.; Rogniaux, Hélène; Jeudy, Alexandra; Barbeyron, Tristan; Médigue, Claudine; Czjzek, Mirjam; Vallenet, David; McBride, Mark J.; Duchaud, Eric; Michel, Gurvan.

In: Nature Communications, Vol. 8, No. 1, 1685, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Préchoux,Aurélie

AU - Thomas,François

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AU - Viart,Benjamin

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AU - Czjzek,Mirjam

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Ficko-Blean E, Préchoux A, Thomas F, Rochat T, Larocque R, Zhu Y et al. Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria. Nature Communications. 2017 Dec 1;8(1). 1685. Available from, DOI: 10.1038/s41467-017-01832-6