Carcinoembryonic antigen (CEA) presentation and specific T cell-priming by human dendritic cells transfected with CEA-mRNA

E. Eppler, H. Horig, H.L. Kaufman, P. Groscurth, Luis Filgueira

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    53 Citations (Scopus)

    Abstract

    The feasibility of dendritic cells (DC) for cancer immunotherapy after transfection by electroporation with mRNA encoding the human carcinoembryonic antigen (CEA) was investigated. Both, total RNA from the CEA colon cancer cell line SW480 and mRNA transcribed in vitro from cDNA3.1-plasmids (pcDNA3.1+/-HisC),with a CEA-insert (M-CEA-mRNA, M-CEA HisC-mRNA) were used. Labelled M-CEA-mRNA was detectable in DC by light and electron microscopy and by fluorescence-activated cell-sorting (FACS) even 15 min after electroporation. Four hours after transfection with M-CEA HisC-mRNA, we detected specific expression of CEA and the histidine-tag by immunofluorescence microscopy and by FACS. CEA-specific T lymphocytes were successfully printed by transfected DC and were able to lyse CEA-expressing target cells, even from the CEA-expressing human colon adenocarcinoma cell line SW480. Thus, DC transfected by electroporation kith CEA-mRNA are valuable tools for the immunotherapy of CEA(+) tumour entities. (C) 2002 Elsevier Science Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)184-193
    JournalEuropean Journal of Cancer
    Volume38
    DOIs
    Publication statusPublished - 2002

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