Brownian dynamics investigation into the conductance state of the MscS channel crystal structure

We suggest that the crystal structure of the mechanosensitive channel of small conductance is in a minimally conductive state rather than being fully activated. Performing Brownian dynamics simulations on the crystal structure show that no ions pass through it. When simulations are conducted on just the transmembrane domain (excluding the cytoplasmic residues 128 to 280) ions are seen to pass through the channel, but the conductance of similar to 30 pS is well below experimentally measured values. The mutation L109S that replaces a pore lining hydrophobic residue with a polar one is found to have little effect on the conductance of the channel. Widening the hydrophobic region of the pore by 2.5 angstrom however, increases the channel conductance to over 200 pS suggesting that only a minimal conformational change is required to gate the pore. (c) 2006 Elsevier B.V All rights reserved.