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Abstract
The type 1 angiotensin II (AngII) receptor (AT1R) transactivates the epidermal growth factor receptor (EGFR), which leads to pathological remodeling of heart, blood vessels and kidney. End-point assays are used as surrogates of EGFR activation, however these downstream readouts are not applicable to live cells, in real-time. Herein, we report the use of a bioluminescence resonance energy transfer (BRET)-based assay to assess recruitment of the EGFR adaptor protein, growth factor receptor-bound protein 2 (Grb2), to the EGFR. In a variety of cell lines, both epidermal growth factor (EGF) and AngII stimulated Grb2 recruitment to EGFR. The BRET assay was used to screen a panel of 9 G protein-coupled receptors (GPCRs) and further developed for other EGFR family members (HER2 and HER3); the AT1R was able to transactivate HER2, but not HER3. Mechanistically, AT1R-mediated ERK1/2 activation was dependent on Gq/11 and EGFR tyrosine kinase activity, whereas the recruitment of Grb2 to the EGFR was independent of Gq/11 and only partially dependent on EGFR tyrosine kinase activity. This Gq/11 independence of EGFR transactivation was confirmed using AT1R mutants and in CRISPR cell lines lacking Gq/11. EGFR transactivation was also apparently independent of β-arrestins. Finally, we used additional BRET-based assays and confocal microscopy to provide evidence that both AngII- and EGF-stimulation promoted AT1R-EGFR heteromerization. In summary, we report an alternative approach to monitoring AT1R-EGFR transactivation in live cells, which provides a more direct and proximal view of this process, including the potential for complexes between the AT1R and EGFR.
Original language | English |
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Pages (from-to) | 232-242 |
Number of pages | 11 |
Journal | Biochemical Pharmacology |
Volume | 158 |
DOIs | |
Publication status | Published - 1 Dec 2018 |
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Dive into the research topics of 'BRET-based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein-independent activation and AT1R-EGFR complexes'. Together they form a unique fingerprint.Projects
- 2 Finished
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Development of technologies to monitor multimolecular complexes
Pfleger, K. (Investigator 01), Rosengren, K. (Investigator 02), Hill, S. (Investigator 03), Abbenante, G. (Investigator 04), Wood, K. (Investigator 05) & Williams, J. (Investigator 06)
ARC Australian Research Council , BMG Labtech Pty Ltd, Dimerix Bioscience Pty Ltd, Promega Corporation
1/01/16 → 2/11/19
Project: Research
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The molecular pharmacology of receptor complexes
Pfleger, K. (Investigator 01)
NHMRC National Health and Medical Research Council
1/01/15 → 31/12/18
Project: Research