Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer

J.M.B. Pang, D.J. Byrne, E.A. Takano, N. Jene, L. Petelin, J. Mckinley, C. Poliness, Christobel Saunders, Donna Taylor, G. Mitchell, S.B. Fox

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    Abstract

    Aim: To investigate the cellular and immunophenotypic basis of mammographic density in women at high risk of breast cancer.
    Methods: Mammograms and targeted breast biopsies were accrued from 24 women at high risk of breast cancer. Mammographic density was classified into Wolfe categories and ranked by increasing density. The histological composition and immunophenotypic profile were quantified from digitized haematoxylin and eosin-stained and immunohistochemically-stained (ERα, ERβ, PgR, HER2, Ki-67, and CD31) slides and correlated to mammographic density.
    Results: Increasing mammographic density was significantly correlated with increased fibrous stroma proportion (rs (22) = 0.5226, p = 0.0088) and significantly inversely associated with adipose tissue proportion (rs (22) = -0.5409, p = 0.0064). Contrary to previous reports, stromal expression of ERα was common (19/20 cases, 95%). There was significantly higher stromal PgR expression in mammographically-dense breasts (p=0.026).
    Conclusions: The proportion of stroma and fat underlies mammographic density in women at high risk of breast cancer. Increased expression of PgR in the stroma of mammographically dense breasts and frequent and unexpected presence of stromal ERα expression raises the possibility that hormone receptor expression in breast stroma may have a role in mediating the effects of exogenous hormonal therapy on mammographic density.
    Original languageEnglish
    Pages (from-to)1-12
    JournalPLoS One
    Volume10
    Issue number6
    DOIs
    Publication statusPublished - 25 Jun 2015

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    breast neoplasms
    breasts
    Breast
    Tissue
    Breast Neoplasms
    Chemical analysis
    Biopsy
    Hematoxylin
    Eosine Yellowish-(YS)
    Fats
    Hormones
    hormone receptors
    tissues
    Breast Density
    Adipose Tissue
    adipose tissue
    biopsy
    therapeutics
    lipids

    Cite this

    Pang, J. M. B., Byrne, D. J., Takano, E. A., Jene, N., Petelin, L., Mckinley, J., ... Fox, S. B. (2015). Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer. PLoS One, 10(6), 1-12. https://doi.org/10.1371/journal.pone.0128861
    Pang, J.M.B. ; Byrne, D.J. ; Takano, E.A. ; Jene, N. ; Petelin, L. ; Mckinley, J. ; Poliness, C. ; Saunders, Christobel ; Taylor, Donna ; Mitchell, G. ; Fox, S.B. / Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer. In: PLoS One. 2015 ; Vol. 10, No. 6. pp. 1-12.
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    abstract = "Aim: To investigate the cellular and immunophenotypic basis of mammographic density in women at high risk of breast cancer. Methods: Mammograms and targeted breast biopsies were accrued from 24 women at high risk of breast cancer. Mammographic density was classified into Wolfe categories and ranked by increasing density. The histological composition and immunophenotypic profile were quantified from digitized haematoxylin and eosin-stained and immunohistochemically-stained (ERα, ERβ, PgR, HER2, Ki-67, and CD31) slides and correlated to mammographic density. Results: Increasing mammographic density was significantly correlated with increased fibrous stroma proportion (rs (22) = 0.5226, p = 0.0088) and significantly inversely associated with adipose tissue proportion (rs (22) = -0.5409, p = 0.0064). Contrary to previous reports, stromal expression of ERα was common (19/20 cases, 95{\%}). There was significantly higher stromal PgR expression in mammographically-dense breasts (p=0.026). Conclusions: The proportion of stroma and fat underlies mammographic density in women at high risk of breast cancer. Increased expression of PgR in the stroma of mammographically dense breasts and frequent and unexpected presence of stromal ERα expression raises the possibility that hormone receptor expression in breast stroma may have a role in mediating the effects of exogenous hormonal therapy on mammographic density.",
    author = "J.M.B. Pang and D.J. Byrne and E.A. Takano and N. Jene and L. Petelin and J. Mckinley and C. Poliness and Christobel Saunders and Donna Taylor and G. Mitchell and S.B. Fox",
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    Pang, JMB, Byrne, DJ, Takano, EA, Jene, N, Petelin, L, Mckinley, J, Poliness, C, Saunders, C, Taylor, D, Mitchell, G & Fox, SB 2015, 'Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer' PLoS One, vol. 10, no. 6, pp. 1-12. https://doi.org/10.1371/journal.pone.0128861

    Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer. / Pang, J.M.B.; Byrne, D.J.; Takano, E.A.; Jene, N.; Petelin, L.; Mckinley, J.; Poliness, C.; Saunders, Christobel; Taylor, Donna; Mitchell, G.; Fox, S.B.

    In: PLoS One, Vol. 10, No. 6, 25.06.2015, p. 1-12.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Breast tissue composition and immunophenotype and its relationship with mammographic density in women at high risk of breast cancer

    AU - Pang, J.M.B.

    AU - Byrne, D.J.

    AU - Takano, E.A.

    AU - Jene, N.

    AU - Petelin, L.

    AU - Mckinley, J.

    AU - Poliness, C.

    AU - Saunders, Christobel

    AU - Taylor, Donna

    AU - Mitchell, G.

    AU - Fox, S.B.

    PY - 2015/6/25

    Y1 - 2015/6/25

    N2 - Aim: To investigate the cellular and immunophenotypic basis of mammographic density in women at high risk of breast cancer. Methods: Mammograms and targeted breast biopsies were accrued from 24 women at high risk of breast cancer. Mammographic density was classified into Wolfe categories and ranked by increasing density. The histological composition and immunophenotypic profile were quantified from digitized haematoxylin and eosin-stained and immunohistochemically-stained (ERα, ERβ, PgR, HER2, Ki-67, and CD31) slides and correlated to mammographic density. Results: Increasing mammographic density was significantly correlated with increased fibrous stroma proportion (rs (22) = 0.5226, p = 0.0088) and significantly inversely associated with adipose tissue proportion (rs (22) = -0.5409, p = 0.0064). Contrary to previous reports, stromal expression of ERα was common (19/20 cases, 95%). There was significantly higher stromal PgR expression in mammographically-dense breasts (p=0.026). Conclusions: The proportion of stroma and fat underlies mammographic density in women at high risk of breast cancer. Increased expression of PgR in the stroma of mammographically dense breasts and frequent and unexpected presence of stromal ERα expression raises the possibility that hormone receptor expression in breast stroma may have a role in mediating the effects of exogenous hormonal therapy on mammographic density.

    AB - Aim: To investigate the cellular and immunophenotypic basis of mammographic density in women at high risk of breast cancer. Methods: Mammograms and targeted breast biopsies were accrued from 24 women at high risk of breast cancer. Mammographic density was classified into Wolfe categories and ranked by increasing density. The histological composition and immunophenotypic profile were quantified from digitized haematoxylin and eosin-stained and immunohistochemically-stained (ERα, ERβ, PgR, HER2, Ki-67, and CD31) slides and correlated to mammographic density. Results: Increasing mammographic density was significantly correlated with increased fibrous stroma proportion (rs (22) = 0.5226, p = 0.0088) and significantly inversely associated with adipose tissue proportion (rs (22) = -0.5409, p = 0.0064). Contrary to previous reports, stromal expression of ERα was common (19/20 cases, 95%). There was significantly higher stromal PgR expression in mammographically-dense breasts (p=0.026). Conclusions: The proportion of stroma and fat underlies mammographic density in women at high risk of breast cancer. Increased expression of PgR in the stroma of mammographically dense breasts and frequent and unexpected presence of stromal ERα expression raises the possibility that hormone receptor expression in breast stroma may have a role in mediating the effects of exogenous hormonal therapy on mammographic density.

    U2 - 10.1371/journal.pone.0128861

    DO - 10.1371/journal.pone.0128861

    M3 - Article

    VL - 10

    SP - 1

    EP - 12

    JO - P L o S One

    JF - P L o S One

    SN - 1932-6203

    IS - 6

    ER -