Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula)

Marie-Claire Castello, Janine Croser, M.M. Lülsdorf, P. Ramankutty, A. Pradhan, Matthew Nelson, Daniel Real

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

© 2013 John Wiley & Sons Ltd. Tedera (Bituminaria bituminosa vars. albomarginata and crassiuscula) is a perennial pasture species with agronomic characters ideally suited to Mediterranean climates. Tedera seed has a period of after-ripening or primary dormancy typically lasting three months, which delays assessment and breeding of elite hybrid varieties. Temperature, chemical and mechanical methods were investigated in conjunction with in vitro culture to circumvent this dormancy period across a range of parental and hybrid genotypes. Temperature treatment of T5 (Tedera accession 5) and T48 (Tedera accession 48) alone was not sufficient to break dormancy (24.0% and 14.7% germination); however, when combined with soaking in gibberellic acid (GA3) and mechanical scarification resulted in 79.7% and 84.3% germination respectively. In an effort to further improve this result for valuable hybrid genotypes, we combined mechanical scarification with removal of seed coat after imbibition and in vitro culture on B5 medium until radicle emergence. This resulted in breaking dormancy from 96% to 100% of parent seeds and 100% of hybrid seeds. Hardening the germinated F1 or F2 seedlings 4 d after first transfer to in vitro culture resulted in 100% survival of plants to soil. This procedure is now used on a routine basis in the Australian tedera breeding programme.
Original languageEnglish
Pages (from-to)365-373
JournalGrass and Forage Science
Volume70
Issue number2
DOIs
Publication statusPublished - 2015

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Bituminaria bituminosa
forage legumes
seed dormancy
dormancy
pasture
in vitro culture
seed
scarification
germination
genotype
seeds
breeding
after-ripening
dormancy breaking
imbibition
ripening
Mediterranean climate
hardening
gibberellic acid
soaking

Cite this

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title = "Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula)",
abstract = "{\circledC} 2013 John Wiley & Sons Ltd. Tedera (Bituminaria bituminosa vars. albomarginata and crassiuscula) is a perennial pasture species with agronomic characters ideally suited to Mediterranean climates. Tedera seed has a period of after-ripening or primary dormancy typically lasting three months, which delays assessment and breeding of elite hybrid varieties. Temperature, chemical and mechanical methods were investigated in conjunction with in vitro culture to circumvent this dormancy period across a range of parental and hybrid genotypes. Temperature treatment of T5 (Tedera accession 5) and T48 (Tedera accession 48) alone was not sufficient to break dormancy (24.0{\%} and 14.7{\%} germination); however, when combined with soaking in gibberellic acid (GA3) and mechanical scarification resulted in 79.7{\%} and 84.3{\%} germination respectively. In an effort to further improve this result for valuable hybrid genotypes, we combined mechanical scarification with removal of seed coat after imbibition and in vitro culture on B5 medium until radicle emergence. This resulted in breaking dormancy from 96{\%} to 100{\%} of parent seeds and 100{\%} of hybrid seeds. Hardening the germinated F1 or F2 seedlings 4 d after first transfer to in vitro culture resulted in 100{\%} survival of plants to soil. This procedure is now used on a routine basis in the Australian tedera breeding programme.",
author = "Marie-Claire Castello and Janine Croser and M.M. L{\"u}lsdorf and P. Ramankutty and A. Pradhan and Matthew Nelson and Daniel Real",
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Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula). / Castello, Marie-Claire; Croser, Janine; Lülsdorf, M.M.; Ramankutty, P.; Pradhan, A.; Nelson, Matthew; Real, Daniel.

In: Grass and Forage Science, Vol. 70, No. 2, 2015, p. 365-373.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula)

AU - Castello, Marie-Claire

AU - Croser, Janine

AU - Lülsdorf, M.M.

AU - Ramankutty, P.

AU - Pradhan, A.

AU - Nelson, Matthew

AU - Real, Daniel

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N2 - © 2013 John Wiley & Sons Ltd. Tedera (Bituminaria bituminosa vars. albomarginata and crassiuscula) is a perennial pasture species with agronomic characters ideally suited to Mediterranean climates. Tedera seed has a period of after-ripening or primary dormancy typically lasting three months, which delays assessment and breeding of elite hybrid varieties. Temperature, chemical and mechanical methods were investigated in conjunction with in vitro culture to circumvent this dormancy period across a range of parental and hybrid genotypes. Temperature treatment of T5 (Tedera accession 5) and T48 (Tedera accession 48) alone was not sufficient to break dormancy (24.0% and 14.7% germination); however, when combined with soaking in gibberellic acid (GA3) and mechanical scarification resulted in 79.7% and 84.3% germination respectively. In an effort to further improve this result for valuable hybrid genotypes, we combined mechanical scarification with removal of seed coat after imbibition and in vitro culture on B5 medium until radicle emergence. This resulted in breaking dormancy from 96% to 100% of parent seeds and 100% of hybrid seeds. Hardening the germinated F1 or F2 seedlings 4 d after first transfer to in vitro culture resulted in 100% survival of plants to soil. This procedure is now used on a routine basis in the Australian tedera breeding programme.

AB - © 2013 John Wiley & Sons Ltd. Tedera (Bituminaria bituminosa vars. albomarginata and crassiuscula) is a perennial pasture species with agronomic characters ideally suited to Mediterranean climates. Tedera seed has a period of after-ripening or primary dormancy typically lasting three months, which delays assessment and breeding of elite hybrid varieties. Temperature, chemical and mechanical methods were investigated in conjunction with in vitro culture to circumvent this dormancy period across a range of parental and hybrid genotypes. Temperature treatment of T5 (Tedera accession 5) and T48 (Tedera accession 48) alone was not sufficient to break dormancy (24.0% and 14.7% germination); however, when combined with soaking in gibberellic acid (GA3) and mechanical scarification resulted in 79.7% and 84.3% germination respectively. In an effort to further improve this result for valuable hybrid genotypes, we combined mechanical scarification with removal of seed coat after imbibition and in vitro culture on B5 medium until radicle emergence. This resulted in breaking dormancy from 96% to 100% of parent seeds and 100% of hybrid seeds. Hardening the germinated F1 or F2 seedlings 4 d after first transfer to in vitro culture resulted in 100% survival of plants to soil. This procedure is now used on a routine basis in the Australian tedera breeding programme.

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