Brainstem organization of efferent projections to the guinea pig cochlea studied using the fluorescent tracers fast blue and diamidino yellow

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    Abstract

    Intracochlear injection of the fluorescent retrograde neuronal tracers fast blue and diamidino yellow was used to investigate the distribution within the brainstem of neurones projecting to the cochlea in the guinea pig. The overall pattern of distribution of cells within the brainstem auditory nuclei was the same for both tracers and was also in broad agreement with recent studies in this species using horse-radish peroxidase as the neuronal tracer. However, the total number of neurones found (mean of 1234 projecting to each cochlea) was significantly greater than that reported using horseradish peroxidase, largely as a result of more small labelled neurones being detected within the lateral superior olivary nucleus ipsilateral to the injected cochlea. The yield of labelled cells was greatest in animals in which care was taken to perfuse the whole length of the cochlear epithelium. After bilateral injections of both tracers, double-labelled cells were found in small numbers within all the large neurone medial system nuclei and the ventral nucleus of the lateral lemniscus. It was concluded that between 1-5% of the medial system neurones project to both cochleas.

    Original languageEnglish
    Pages (from-to)449-457
    Number of pages9
    JournalExperimental Brain Research
    Volume66
    Issue number3
    DOIs
    Publication statusPublished - 1 May 1987

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    Cochlea
    Brain Stem
    Guinea Pigs
    Neurons
    Raphanus
    Injections
    Horseradish Peroxidase
    Peroxidase
    Horses
    Epithelium
    diamidino compound 253-50
    diamidino yellow

    Cite this

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    title = "Brainstem organization of efferent projections to the guinea pig cochlea studied using the fluorescent tracers fast blue and diamidino yellow",
    abstract = "Intracochlear injection of the fluorescent retrograde neuronal tracers fast blue and diamidino yellow was used to investigate the distribution within the brainstem of neurones projecting to the cochlea in the guinea pig. The overall pattern of distribution of cells within the brainstem auditory nuclei was the same for both tracers and was also in broad agreement with recent studies in this species using horse-radish peroxidase as the neuronal tracer. However, the total number of neurones found (mean of 1234 projecting to each cochlea) was significantly greater than that reported using horseradish peroxidase, largely as a result of more small labelled neurones being detected within the lateral superior olivary nucleus ipsilateral to the injected cochlea. The yield of labelled cells was greatest in animals in which care was taken to perfuse the whole length of the cochlear epithelium. After bilateral injections of both tracers, double-labelled cells were found in small numbers within all the large neurone medial system nuclei and the ventral nucleus of the lateral lemniscus. It was concluded that between 1-5{\%} of the medial system neurones project to both cochleas.",
    keywords = "Cochlea, Efferents, Fluorescent tracers, Guinea pig",
    author = "D. Robertson and Cole, {K. S.} and Harvey, {A. R.}",
    year = "1987",
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    TY - JOUR

    T1 - Brainstem organization of efferent projections to the guinea pig cochlea studied using the fluorescent tracers fast blue and diamidino yellow

    AU - Robertson, D.

    AU - Cole, K. S.

    AU - Harvey, A. R.

    PY - 1987/5/1

    Y1 - 1987/5/1

    N2 - Intracochlear injection of the fluorescent retrograde neuronal tracers fast blue and diamidino yellow was used to investigate the distribution within the brainstem of neurones projecting to the cochlea in the guinea pig. The overall pattern of distribution of cells within the brainstem auditory nuclei was the same for both tracers and was also in broad agreement with recent studies in this species using horse-radish peroxidase as the neuronal tracer. However, the total number of neurones found (mean of 1234 projecting to each cochlea) was significantly greater than that reported using horseradish peroxidase, largely as a result of more small labelled neurones being detected within the lateral superior olivary nucleus ipsilateral to the injected cochlea. The yield of labelled cells was greatest in animals in which care was taken to perfuse the whole length of the cochlear epithelium. After bilateral injections of both tracers, double-labelled cells were found in small numbers within all the large neurone medial system nuclei and the ventral nucleus of the lateral lemniscus. It was concluded that between 1-5% of the medial system neurones project to both cochleas.

    AB - Intracochlear injection of the fluorescent retrograde neuronal tracers fast blue and diamidino yellow was used to investigate the distribution within the brainstem of neurones projecting to the cochlea in the guinea pig. The overall pattern of distribution of cells within the brainstem auditory nuclei was the same for both tracers and was also in broad agreement with recent studies in this species using horse-radish peroxidase as the neuronal tracer. However, the total number of neurones found (mean of 1234 projecting to each cochlea) was significantly greater than that reported using horseradish peroxidase, largely as a result of more small labelled neurones being detected within the lateral superior olivary nucleus ipsilateral to the injected cochlea. The yield of labelled cells was greatest in animals in which care was taken to perfuse the whole length of the cochlear epithelium. After bilateral injections of both tracers, double-labelled cells were found in small numbers within all the large neurone medial system nuclei and the ventral nucleus of the lateral lemniscus. It was concluded that between 1-5% of the medial system neurones project to both cochleas.

    KW - Cochlea

    KW - Efferents

    KW - Fluorescent tracers

    KW - Guinea pig

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    DO - 10.1007/BF00270677

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