TY - JOUR
T1 - Boron inhibits cadmium uptake in wheat (Triticum aestivum) by regulating gene expression
AU - Qin, Shiyu
AU - Liu, Hongen
AU - Rengel, Zed
AU - Gao, Wei
AU - Nie, Zhaojun
AU - Li, Chang
AU - Hou, Mingyang
AU - Cheng, Jin
AU - Zhao, Peng
PY - 2020/8
Y1 - 2020/8
N2 - Various nutrients (Mg, Zn, Fe, Mn, Si, etc.) can supress cadmium (Cd) uptake and alleviate Cd toxicity, but the mechanisms are not the same. In this study, the molecular mechanism governing the effects of boron (B) on uptake of Cd in hydroponically grown wheat was characterized. As compared to control (0 μM Cd), B concentration per plant decreased by 22% and 29% under 5 μM Cd and 50 μM Cd treatment respectively. In addition, B application decreased Cd concentration and accumulation in whole wheat. Correlation analysis of different elements show that there was a highly negative correlation between concentrations of B and Cd (r = −0.854 with significant correlation) in wheat. Additionally, 16,543 differentially expressed genes (DEGs) (7666 up- and 8877 down-regulated) were detected between 0 and 5 μM Cd treatments in wheat roots by transcriptome sequencing. Gene ontology functional category and Kyoto encyclopedia of genes and genomes pathway analyses indicated that the DEGs were involved in biological process, cellular component, and molecular function. Five highly homologous genes to Cd transporters were identified; these genes were involved in metal ion binding, transmembrane ion transport, and protein transport. According to the qRT-PCR results, expression of all these genes was down-regulated in the 462 μM of B treatment compared with the 46.2 μM of B treatment regardless of the Cd treatments (0.5 or 5 μM Cd). These results suggest that B is an inhibitor of Cd uptake, and the down-regulation of five highly homologous genes could be associated with decreased uptake of Cd after B application.
AB - Various nutrients (Mg, Zn, Fe, Mn, Si, etc.) can supress cadmium (Cd) uptake and alleviate Cd toxicity, but the mechanisms are not the same. In this study, the molecular mechanism governing the effects of boron (B) on uptake of Cd in hydroponically grown wheat was characterized. As compared to control (0 μM Cd), B concentration per plant decreased by 22% and 29% under 5 μM Cd and 50 μM Cd treatment respectively. In addition, B application decreased Cd concentration and accumulation in whole wheat. Correlation analysis of different elements show that there was a highly negative correlation between concentrations of B and Cd (r = −0.854 with significant correlation) in wheat. Additionally, 16,543 differentially expressed genes (DEGs) (7666 up- and 8877 down-regulated) were detected between 0 and 5 μM Cd treatments in wheat roots by transcriptome sequencing. Gene ontology functional category and Kyoto encyclopedia of genes and genomes pathway analyses indicated that the DEGs were involved in biological process, cellular component, and molecular function. Five highly homologous genes to Cd transporters were identified; these genes were involved in metal ion binding, transmembrane ion transport, and protein transport. According to the qRT-PCR results, expression of all these genes was down-regulated in the 462 μM of B treatment compared with the 46.2 μM of B treatment regardless of the Cd treatments (0.5 or 5 μM Cd). These results suggest that B is an inhibitor of Cd uptake, and the down-regulation of five highly homologous genes could be associated with decreased uptake of Cd after B application.
KW - Analysis
KW - Boron
KW - Cadmium
KW - Gene expression
KW - RNA-Seq
KW - Uptake
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=85084668099&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2020.110522
DO - 10.1016/j.plantsci.2020.110522
M3 - Article
C2 - 32563461
AN - SCOPUS:85084668099
VL - 297
JO - Plant Science
JF - Plant Science
SN - 0168-9452
M1 - 110522
ER -