Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

Nathan D. Tivendale; Sandra E. Davidson; Noel W. Davies; Jason A. Smith; Marion Dalmais; Abdelhafid I. Bendahmane; Laura J. Quittenden; Lily Sutton; Raj K. Bala; Christine Le Signor; Richard Thompson; James Horne; James B. Reid; John J. Ross

doi:10.1104/pp.112.198457

Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

Nathan D. Tivendale, Sandra E. Davidson, Noel W. Davies, Jason A. Smith, Marion Dalmais, Abdelhafid I. Bendahmane, Laura J. Quittenden, Lily Sutton, Raj K. Bala, Christine Le Signor, Richard Thompson, James Horne, James B. Reid, John J. Ross

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.

Original language	English
Pages (from-to)	1055-1063
Number of pages	9
Journal	Plant Physiology
Volume	159
Issue number	3
DOIs	https://doi.org/10.1104/pp.112.198457
Publication status	Published - Jul 2012
Externally published	Yes

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@article{22f62252a54b4b8e9766eba26b6b0250,

title = "Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid",

abstract = "Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.",

keywords = "PLANT DEVELOPMENT, PEA FRUIT, ARABIDOPSIS, PATHWAY, TRYPTOPHAN, GROWTH, MAIZE",

author = "Tivendale, {Nathan D.} and Davidson, {Sandra E.} and Davies, {Noel W.} and Smith, {Jason A.} and Marion Dalmais and Bendahmane, {Abdelhafid I.} and Quittenden, {Laura J.} and Lily Sutton and Bala, {Raj K.} and {Le Signor}, Christine and Richard Thompson and James Horne and Reid, {James B.} and Ross, {John J.}",

year = "2012",

month = jul,

doi = "10.1104/pp.112.198457",

language = "English",

volume = "159",

pages = "1055--1063",

journal = "Plant Physiology",

issn = "0032-0889",

publisher = "American Society of Plant Biologists",

number = "3",

}

TY - JOUR

T1 - Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

AU - Tivendale, Nathan D.

AU - Davidson, Sandra E.

AU - Davies, Noel W.

AU - Smith, Jason A.

AU - Dalmais, Marion

AU - Bendahmane, Abdelhafid I.

AU - Quittenden, Laura J.

AU - Sutton, Lily

AU - Bala, Raj K.

AU - Le Signor, Christine

AU - Thompson, Richard

AU - Horne, James

AU - Reid, James B.

AU - Ross, John J.

PY - 2012/7

Y1 - 2012/7

N2 - Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.

AB - Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.

KW - PLANT DEVELOPMENT

KW - PEA FRUIT

KW - ARABIDOPSIS

KW - PATHWAY

KW - TRYPTOPHAN

KW - GROWTH

KW - MAIZE

U2 - 10.1104/pp.112.198457

DO - 10.1104/pp.112.198457

M3 - Article

SN - 0032-0889

VL - 159

SP - 1055

EP - 1063

JO - Plant Physiology

JF - Plant Physiology

IS - 3

ER -

Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

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