Biophysical detection of diversity and bias in GPCR function

Werner Jaeger, Stephen Armstrong, S.J. Hill, Kevin Pfleger

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Guanine nucleotide binding protein (G protein)-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin, and other receptors. Elements of these complexes may interact constitutively or dynamically, dependent upon factors such as ligand binding, phosphorylation, and dephosphorylation. They may also be allosterically modulated by other proteins in a manner that changes temporally and spatially within the cell. Elucidating how these complexes function has been greatly enhanced by biophysical technologies that are able to monitor proximity and/or binding, often in real time and in live cells. These include resonance energy transfer approaches such as bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET). Furthermore, the use of fluorescent ligands has enabled novel insights into allosteric interactions between GPCRs. Consequently, biophysical approaches are helping to unlock the amazing diversity and bias in G protein-coupled receptor signaling. © 2014 Jaeger, Armstrong, Hill and Pfleger.
Original languageEnglish
Pages (from-to)1-11
JournalFrontiers in Endocrinology
Volume5
Issue numberMAR
DOIs
Publication statusPublished - 2014

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