Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)

Cesar E. Fribourg, Adrian J. Gibbs, Ian P. Adams, Neil Boonham, Roger A.C. Jones

Research output: Contribution to journalArticle

Abstract

In 1976, a virus with flexuous, filamentous virions typical of the family Potyviridae was isolated from symptomatic pepino (Solanum muricatum) plants growing in two valleys in Peru's coastal desert region. In 2014, a virus with similar-shaped virions was isolated from asymptomatic fruits obtained from pepino plants growing in six coastal valleys and a valley in Peru's Andean highlands. Both were identified subsequently as Wild potato mosaic virus (WPMV) by serology or high-throughput sequencing (HTS). The symptoms caused by two old and seven new isolates from pepino were examined in indicator plants. Infected solanaceous hosts varied considerably in their sensitivities to infection and individual isolates varied greatly in virulence. All seven new isolates caused quick death of infected Nicotiana benthamiana plants and more than half of them killed infected plants of Physalis floridana and S. chancayense. These three species were the most sensitive to infection. The most virulent isolate was found to be BA because it killed five of eight solanaceous host species whereas CA was the least severe because it only killed N. benthamiana. Using HTS, complete genomic sequences of six isolates were obtained, with one isolate (FE) showing evidence of recombination. The distances between individual WPMV isolates in phylogenetic trees and the geographical distances between their collection sites were found to be unrelated. The individual WPMV isolates displayed nucleotide sequence identities of 80.9-99.8%, whereas the most closely related virus, Potato virus V (PVV), was around 75% identical to WPMV. WPMV, PVV, and Peru tomato virus formed clusters of similar phylogenetic diversity, and were found to be distinct but related viruses within the overall Potato virus Y lineage. WPMV infection seems widespread and of likely economic significance to pepino producers in Peru's coastal valleys. Because it constitutes the fifth virus found infecting pepino and this crop is entirely vegetatively propagated, development of healthy pepino stock programs is advocated.

Original languageEnglish
Pages (from-to)1746-1756
Number of pages11
JournalPlant Disease
Volume103
Issue number7
DOIs
Publication statusPublished - 1 Jul 2019

Fingerprint

Solanum muricatum
Potato virus V
valleys
viruses
Peru
Nicotiana benthamiana
virion
Physalis pubescens
infection
Potyviridae
Potato virus Y
phylogeny
Wild potato mosaic virus
signs and symptoms (plants)
indicator species
deserts
highlands
virulence
death
genomics

Cite this

Fribourg, Cesar E. ; Gibbs, Adrian J. ; Adams, Ian P. ; Boonham, Neil ; Jones, Roger A.C. / Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum). In: Plant Disease. 2019 ; Vol. 103, No. 7. pp. 1746-1756.
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Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum). / Fribourg, Cesar E.; Gibbs, Adrian J.; Adams, Ian P.; Boonham, Neil; Jones, Roger A.C.

In: Plant Disease, Vol. 103, No. 7, 01.07.2019, p. 1746-1756.

Research output: Contribution to journalArticle

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AU - Fribourg, Cesar E.

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