Biofilm detection in natural unconsolidated porous media using a low-field magnetic resonance system

A.B. Sanderlin, Sarah Vogt, E. Grunewald, B.A. Bergin, S.L. Codd

    Research output: Contribution to journalArticle

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    Abstract

    The extent to which T2 relaxation measurements can be used to determine biofouling in several natural geological sand media using a low-field (275 kHz, 6.5 mT) NMR system has been demonstrated. It has been previously shown that, at high laboratory strength fields (300 MHz, 7 T), T2 techniques can be used as a bioassay to confirm the growth of biofilm inside opaque porous media with low magnetic susceptibilities such as borosilicate or soda lime glass beads. Additionally decreases in T2 can be associated with intact biofilm as opposed to degraded biofilm material. However, in natural geological media, the strong susceptibility gradients generated at high fields dominated the T2 relaxation time distributions and biofilm growth could not be reliably detected. Samples studied included Bacillus mojavensis biofilm in several sand types, as well as alginate solution and alginate gel in several sand types. One of the sand types was highly magnetic. Data was collected with a low-field (275 kHz, 6.5 mT) benchtop NMR system using a CPMG sequence with an echo time of 1.25 ms providing the ability to detect signals with T2 greater than 1 ms. Data presented here clearly demonstrate that biofilm can be reliably detected and monitored in highly magnetically susceptible geological samples using a low-field NMR spectrometer indicating that low-field NMR could be viable as a biofilm sensor at bioremedation sites. © 2012 American Chemical Society.
    Original languageEnglish
    Pages (from-to)987-992
    JournalEnvironmental Science and Technology
    Volume47
    Issue number2
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Biofilms
    Magnetic resonance
    biofilm
    Porous materials
    porous medium
    magnetic field
    nuclear magnetic resonance
    Sand
    Nuclear magnetic resonance
    sand
    alginate
    Biofouling
    biofouling
    Bioassay
    Bacilli
    magnetic susceptibility
    detection
    Magnetic susceptibility
    Relaxation time
    lime

    Cite this

    Sanderlin, A.B. ; Vogt, Sarah ; Grunewald, E. ; Bergin, B.A. ; Codd, S.L. / Biofilm detection in natural unconsolidated porous media using a low-field magnetic resonance system. In: Environmental Science and Technology. 2013 ; Vol. 47, No. 2. pp. 987-992.
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    abstract = "The extent to which T2 relaxation measurements can be used to determine biofouling in several natural geological sand media using a low-field (275 kHz, 6.5 mT) NMR system has been demonstrated. It has been previously shown that, at high laboratory strength fields (300 MHz, 7 T), T2 techniques can be used as a bioassay to confirm the growth of biofilm inside opaque porous media with low magnetic susceptibilities such as borosilicate or soda lime glass beads. Additionally decreases in T2 can be associated with intact biofilm as opposed to degraded biofilm material. However, in natural geological media, the strong susceptibility gradients generated at high fields dominated the T2 relaxation time distributions and biofilm growth could not be reliably detected. Samples studied included Bacillus mojavensis biofilm in several sand types, as well as alginate solution and alginate gel in several sand types. One of the sand types was highly magnetic. Data was collected with a low-field (275 kHz, 6.5 mT) benchtop NMR system using a CPMG sequence with an echo time of 1.25 ms providing the ability to detect signals with T2 greater than 1 ms. Data presented here clearly demonstrate that biofilm can be reliably detected and monitored in highly magnetically susceptible geological samples using a low-field NMR spectrometer indicating that low-field NMR could be viable as a biofilm sensor at bioremedation sites. {\circledC} 2012 American Chemical Society.",
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    Biofilm detection in natural unconsolidated porous media using a low-field magnetic resonance system. / Sanderlin, A.B.; Vogt, Sarah; Grunewald, E.; Bergin, B.A.; Codd, S.L.

    In: Environmental Science and Technology, Vol. 47, No. 2, 2013, p. 987-992.

    Research output: Contribution to journalArticle

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