TY - JOUR
T1 - Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles
AU - Navone, Laura
AU - Moffitt, Kaylee
AU - Johnston, Wayne A.
AU - Mercer, Tim
AU - Cooper, Crystal
AU - Spann, Kirsten
AU - Speight, Robert E.
N1 - Funding Information:
The authors thank Dr Christopher Doropoulos (Commonwealth Scientific and Industrial Research Organisation, Australia) for his help on absorption data model fit. We also thank Professor David Baker from the University of Washington for kindly providing the antiviral peptide sequences prior to publication. This work was funded by the Centre for Agriculture and Bioeconomy and the Centre for a Waste Free World, Queensland University of Technology. We also acknowledge the Central Analytical Research Facility, operated and funded by Queensland University of Technology (QUT), and the financial support of the Australian Research Council Centre of Excellence in Synthetic Biology. Part of the figures was created with BioRender.com. L.N. is supported by QUT and the CSIRO Synthetic Biology Future Science Platform.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - The use of personal protective equipment (PPE), face masks and ventilation are key strategies to control the transmission of respiratory viruses. However, most PPE provides physical protection that only partially prevents the transmission of viral particles. Here, we develop textiles with integrated peptide binders that capture viral particles. We fuse peptides capable of binding the receptor domain of the spike protein on the SARS-CoV-2 capsid to the cellulose-binding domain from the Trichoderma reesei cellobiohydrolase II protein. The hybrid peptides can be attached to the cellulose fibres in cotton and capture SARS-CoV-2 viral particles with high affinity. The resulting bioengineered cotton captures 114,000 infective virus particles per cm2 and reduces onwards SARS-CoV-2 infection of cells by 500-fold. The hybrid peptides could be easily modified to capture and control the spread of other infectious pathogens or for attachment to different materials. We anticipate the use of bioengineered protective textiles in PPE, facemasks, ventilation, and furnishings will provide additional protection to the airborne or fomite transmission of viruses.
AB - The use of personal protective equipment (PPE), face masks and ventilation are key strategies to control the transmission of respiratory viruses. However, most PPE provides physical protection that only partially prevents the transmission of viral particles. Here, we develop textiles with integrated peptide binders that capture viral particles. We fuse peptides capable of binding the receptor domain of the spike protein on the SARS-CoV-2 capsid to the cellulose-binding domain from the Trichoderma reesei cellobiohydrolase II protein. The hybrid peptides can be attached to the cellulose fibres in cotton and capture SARS-CoV-2 viral particles with high affinity. The resulting bioengineered cotton captures 114,000 infective virus particles per cm2 and reduces onwards SARS-CoV-2 infection of cells by 500-fold. The hybrid peptides could be easily modified to capture and control the spread of other infectious pathogens or for attachment to different materials. We anticipate the use of bioengineered protective textiles in PPE, facemasks, ventilation, and furnishings will provide additional protection to the airborne or fomite transmission of viruses.
UR - http://www.scopus.com/inward/record.url?scp=85135982097&partnerID=8YFLogxK
U2 - 10.1038/s43246-022-00278-8
DO - 10.1038/s43246-022-00278-8
M3 - Article
C2 - 35991518
SN - 2662-4443
VL - 3
JO - Communications Materials
JF - Communications Materials
IS - 1
M1 - 54
ER -