TY - JOUR
T1 - Biocompatibility of glycerol monooleate nanoparticles as tested on inner ear cells
AU - Simoni, Edi
AU - Valente, Filippo
AU - Boge, Lukas
AU - Eriksson, Mimmi
AU - Gentilin, Erica
AU - Candito, Mariarita
AU - Cazzador, Diego
AU - Astolfi, Laura
PY - 2019/12/15
Y1 - 2019/12/15
N2 - Sensorineural hearing loss due to aging, noise exposure, trauma or drug ototoxicity is irreversible because cochlear hair cells and neurons cannot regenerate. Recently, therapeutic strategies involving nanoparticles have been developed as innovative drug delivery systems. Thermodynamically stable liquid crystalline nanoparticles based on the polar lipid glycerol monooleate (GMO NP, cubosomes), nontoxic and able to encapsulate both hydrophilic and hydrophobic compounds, were produced and tested for biocompatibility in an immortalized Organ of Corti derived cell line (OC-k3), through cell viability and cytomorphological assays, and Western blot expression profiles of apoptotic markers. Overall, the GMO NP were biocompatible in OC-k3 at the doses and time tested, supporting previous data obtained in a neuronal cell line (PC12). The results encourage further tests on GMO NP-mediated drug release with improved target specificity and could be useful to develop innovative therapies against sensorineural hearing loss.
AB - Sensorineural hearing loss due to aging, noise exposure, trauma or drug ototoxicity is irreversible because cochlear hair cells and neurons cannot regenerate. Recently, therapeutic strategies involving nanoparticles have been developed as innovative drug delivery systems. Thermodynamically stable liquid crystalline nanoparticles based on the polar lipid glycerol monooleate (GMO NP, cubosomes), nontoxic and able to encapsulate both hydrophilic and hydrophobic compounds, were produced and tested for biocompatibility in an immortalized Organ of Corti derived cell line (OC-k3), through cell viability and cytomorphological assays, and Western blot expression profiles of apoptotic markers. Overall, the GMO NP were biocompatible in OC-k3 at the doses and time tested, supporting previous data obtained in a neuronal cell line (PC12). The results encourage further tests on GMO NP-mediated drug release with improved target specificity and could be useful to develop innovative therapies against sensorineural hearing loss.
KW - Apoptosis
KW - Cell cycle
KW - Cubosome
KW - OC-k3 cells
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85075399346&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2019.118788
DO - 10.1016/j.ijpharm.2019.118788
M3 - Article
C2 - 31676341
AN - SCOPUS:85075399346
SN - 0378-5173
VL - 572
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
M1 - 118788
ER -