Bean α-Amylase Inhibitors in Transgenic Peas Inhibit Development of Pea Weevil Larvaeα

M.J.D. Sousa-Majer, D.C. Hardie, Neil Turner, T.J.V. Higgins

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    This glasshouse study used an improved larval measurement procedure to evaluate the impact of transgenic pea, Pisum sativum L., seeds expressing a-amylase inhibitor (AI) -1 or -2 proteins on pea weevil, Bruchus pisorum L. Seeds of transgenic 'Laura' and 'Greenfeast' peas expressing alpha-(AI)-1 reduced pea weevil survival by 93-98%. Larval mortality occurred at an early instar. Conversely, in nontransgenic cultivars, approximate to 98-99% of the pea weevils emerged as adults. By measuring the bead capsule size, we determined that larvae died at the first to early third instar in alpha-(AI)-1 transgenic peas, indicating that this inhibitor is highly effective in controlling this insect. By contrast, transgenic Laura and 'Dundale' expressing alpha- (AI) -2 did not affect pea weevil survival, but they did delay larval development. After 77 d of development, the head capsule size indicated that the larvae were still at the third instar stage in transgenic alpha- (AI) -2 peas, whereas adult bruchids had developed in the nontransgenic peas.
    Original languageEnglish
    Pages (from-to)1416-1422
    JournalJournal of Economic Entomology
    Volume100
    Issue number4
    DOIs
    Publication statusPublished - 2007

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