Bacillus subtilis and surfactant amendments for the breakdown of soil water repellency in a sandy soil

Mary Anne Lowe, Falko Mathes, Meng Heng Loke, Gavan McGrath, Daniel V. Murphy, Matthias Leopold

Research output: Contribution to journalArticle

Abstract

Soil water repellency (SWR) is an agricultural concern as it limits plant available water, leading to decreased crop yields at times when a drying climate and expanding population place increasing pressure on food production. Here we assessed a microbial inoculation that secretes biosurfactants and lipolytic enzymes and a chemical surfactant's ability to mitigate SWR. We applied either Bacillus subtilis (Gm), surfactant (Gs), Bacillus subtilis with surfactant (Gms) or no treatment (G) to a gamma-irradiated, naturally water-repellent, agricultural soil, with the same soil as an un-sterilised, untreated control (C). Soil water content and the persistence of SWR in a water-repellent core (4 × 4 × 3 cm) were measured during 19 days of repeated soil wetting and drying. Meso-scale, 3D electrical resistivity tomography (ERT) was developed for the continuous, non-destructive measurement of the patterns and volume of water content in the soil, however, effectiveness was limited as the electrical current (5 mA) restricted microbial activity. Destructive measurements of soil water content and potential SWR were conducted in 1 cm soil layers throughout each core. The persistence of SWR decreased over time in all treatments apart from treatment G. SWR was removed by day 12 within the Gm treatment, and was not exhibited immediately after wetting in treatments Gs and Gms. The Gs treatment also increased water content immediately while treatment Gm increased water content after eight days of incubation, with soil saturated by day 19 of the incubation. Persistence of SWR significantly varied with depth, with the highest persistence in the 1–2 cm soil layer. Our findings illustrate that in order to remove SWR, without the effect of chemical surfactants, first microbial degradation of the molecules attributing to SWR has to occur. This indicates a biological mechanism as the driver for non-reversible SWR breakdown rather than a solely physical process.

Original languageEnglish
Pages (from-to)108-118
Number of pages11
JournalGeoderma
Volume344
DOIs
Publication statusPublished - 15 Jun 2019

Fingerprint

surfactants
Bacillus subtilis
sandy soil
sandy soils
surfactant
soil water
water content
persistence
soil water content
soil
repellents
wetting
drying
incubation
biosurfactants
electric current
electrical resistance
soil water potential
plant available water
tomography

Cite this

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title = "Bacillus subtilis and surfactant amendments for the breakdown of soil water repellency in a sandy soil",
abstract = "Soil water repellency (SWR) is an agricultural concern as it limits plant available water, leading to decreased crop yields at times when a drying climate and expanding population place increasing pressure on food production. Here we assessed a microbial inoculation that secretes biosurfactants and lipolytic enzymes and a chemical surfactant's ability to mitigate SWR. We applied either Bacillus subtilis (Gm), surfactant (Gs), Bacillus subtilis with surfactant (Gms) or no treatment (G) to a gamma-irradiated, naturally water-repellent, agricultural soil, with the same soil as an un-sterilised, untreated control (C). Soil water content and the persistence of SWR in a water-repellent core (4 × 4 × 3 cm) were measured during 19 days of repeated soil wetting and drying. Meso-scale, 3D electrical resistivity tomography (ERT) was developed for the continuous, non-destructive measurement of the patterns and volume of water content in the soil, however, effectiveness was limited as the electrical current (5 mA) restricted microbial activity. Destructive measurements of soil water content and potential SWR were conducted in 1 cm soil layers throughout each core. The persistence of SWR decreased over time in all treatments apart from treatment G. SWR was removed by day 12 within the Gm treatment, and was not exhibited immediately after wetting in treatments Gs and Gms. The Gs treatment also increased water content immediately while treatment Gm increased water content after eight days of incubation, with soil saturated by day 19 of the incubation. Persistence of SWR significantly varied with depth, with the highest persistence in the 1–2 cm soil layer. Our findings illustrate that in order to remove SWR, without the effect of chemical surfactants, first microbial degradation of the molecules attributing to SWR has to occur. This indicates a biological mechanism as the driver for non-reversible SWR breakdown rather than a solely physical process.",
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Bacillus subtilis and surfactant amendments for the breakdown of soil water repellency in a sandy soil. / Lowe, Mary Anne; Mathes, Falko; Loke, Meng Heng; McGrath, Gavan; Murphy, Daniel V.; Leopold, Matthias.

In: Geoderma, Vol. 344, 15.06.2019, p. 108-118.

Research output: Contribution to journalArticle

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