Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies

M.S.G. Kwa, I.N. Van Schaik, R.R. Dejonge, A. Brand, Luba Kalaydjieva, N. Van Belzen, M. Vermeulen, F. Baas

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Inflammatory demyelinating neuropathies are characterized by a loss of peripheral nerve myelin. Myelin breakdown is thought to result from an autoimmune reaction towards nerve components. Schwann cells play a crucial role in the synthesis and maintenance of peripheral nerve myelin. An immune attack targeting Schwann cells could therefore affect myelin integrity, leading to disease. We studied the reactivity of sera from patients with Guillain-Barre syndrome (GBS) and chronic inflammatory demyelinating polyneuropathy (CIDP) towards Schwann cells using immunofluorescence microscopy. We found 24% of the GBS (56 out of 233) and 26% of the CIDP (12 out of 46) patients to have circulating immunoglobulin G autoantibodies against proliferating, non-myelinating human Schwann cells. In contrast, healthy donors showed positive staining in only two out of 34 sera. No reaction was found with sera from patients with non-inflammatory neurological disorders. Immunofluorescence was localized at the distal tips (leading lamella) of the Schwann cell processes. Distal tips of neurites (nerve-growth-cones) of in vitro differentiated non-myelinated hNT2 neurons also stained strongly. GBS and CIDP serum immunoreactivity was also observed in teased nerve fibre preparations. These data suggest that, at least part of the immunoreactivity is not directed against myelin, but towards non-myelin proteins and epitopes possibly involved in Schwann cell-axon interaction.
Original languageEnglish
Pages (from-to)361-375
JournalBrain
Volume126
Issue number2
DOIs
Publication statusPublished - 2003

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Schwann Cells
Myelin Sheath
Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Guillain-Barre Syndrome
Serum
Peripheral Nerves
Growth Cones
Neurites
Nervous System Diseases
Nerve Fibers
Fluorescence Microscopy
Cell Communication
Autoantibodies
Fluorescent Antibody Technique
Axons
Epitopes
Immunoglobulin G
Maintenance
Tissue Donors
Staining and Labeling

Cite this

Kwa, M. S. G., Van Schaik, I. N., Dejonge, R. R., Brand, A., Kalaydjieva, L., Van Belzen, N., ... Baas, F. (2003). Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies. Brain, 126(2), 361-375. https://doi.org/10.1093/brain/awg030
Kwa, M.S.G. ; Van Schaik, I.N. ; Dejonge, R.R. ; Brand, A. ; Kalaydjieva, Luba ; Van Belzen, N. ; Vermeulen, M. ; Baas, F. / Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies. In: Brain. 2003 ; Vol. 126, No. 2. pp. 361-375.
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abstract = "Inflammatory demyelinating neuropathies are characterized by a loss of peripheral nerve myelin. Myelin breakdown is thought to result from an autoimmune reaction towards nerve components. Schwann cells play a crucial role in the synthesis and maintenance of peripheral nerve myelin. An immune attack targeting Schwann cells could therefore affect myelin integrity, leading to disease. We studied the reactivity of sera from patients with Guillain-Barre syndrome (GBS) and chronic inflammatory demyelinating polyneuropathy (CIDP) towards Schwann cells using immunofluorescence microscopy. We found 24{\%} of the GBS (56 out of 233) and 26{\%} of the CIDP (12 out of 46) patients to have circulating immunoglobulin G autoantibodies against proliferating, non-myelinating human Schwann cells. In contrast, healthy donors showed positive staining in only two out of 34 sera. No reaction was found with sera from patients with non-inflammatory neurological disorders. Immunofluorescence was localized at the distal tips (leading lamella) of the Schwann cell processes. Distal tips of neurites (nerve-growth-cones) of in vitro differentiated non-myelinated hNT2 neurons also stained strongly. GBS and CIDP serum immunoreactivity was also observed in teased nerve fibre preparations. These data suggest that, at least part of the immunoreactivity is not directed against myelin, but towards non-myelin proteins and epitopes possibly involved in Schwann cell-axon interaction.",
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Kwa, MSG, Van Schaik, IN, Dejonge, RR, Brand, A, Kalaydjieva, L, Van Belzen, N, Vermeulen, M & Baas, F 2003, 'Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies' Brain, vol. 126, no. 2, pp. 361-375. https://doi.org/10.1093/brain/awg030

Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies. / Kwa, M.S.G.; Van Schaik, I.N.; Dejonge, R.R.; Brand, A.; Kalaydjieva, Luba; Van Belzen, N.; Vermeulen, M.; Baas, F.

In: Brain, Vol. 126, No. 2, 2003, p. 361-375.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies

AU - Kwa, M.S.G.

AU - Van Schaik, I.N.

AU - Dejonge, R.R.

AU - Brand, A.

AU - Kalaydjieva, Luba

AU - Van Belzen, N.

AU - Vermeulen, M.

AU - Baas, F.

PY - 2003

Y1 - 2003

N2 - Inflammatory demyelinating neuropathies are characterized by a loss of peripheral nerve myelin. Myelin breakdown is thought to result from an autoimmune reaction towards nerve components. Schwann cells play a crucial role in the synthesis and maintenance of peripheral nerve myelin. An immune attack targeting Schwann cells could therefore affect myelin integrity, leading to disease. We studied the reactivity of sera from patients with Guillain-Barre syndrome (GBS) and chronic inflammatory demyelinating polyneuropathy (CIDP) towards Schwann cells using immunofluorescence microscopy. We found 24% of the GBS (56 out of 233) and 26% of the CIDP (12 out of 46) patients to have circulating immunoglobulin G autoantibodies against proliferating, non-myelinating human Schwann cells. In contrast, healthy donors showed positive staining in only two out of 34 sera. No reaction was found with sera from patients with non-inflammatory neurological disorders. Immunofluorescence was localized at the distal tips (leading lamella) of the Schwann cell processes. Distal tips of neurites (nerve-growth-cones) of in vitro differentiated non-myelinated hNT2 neurons also stained strongly. GBS and CIDP serum immunoreactivity was also observed in teased nerve fibre preparations. These data suggest that, at least part of the immunoreactivity is not directed against myelin, but towards non-myelin proteins and epitopes possibly involved in Schwann cell-axon interaction.

AB - Inflammatory demyelinating neuropathies are characterized by a loss of peripheral nerve myelin. Myelin breakdown is thought to result from an autoimmune reaction towards nerve components. Schwann cells play a crucial role in the synthesis and maintenance of peripheral nerve myelin. An immune attack targeting Schwann cells could therefore affect myelin integrity, leading to disease. We studied the reactivity of sera from patients with Guillain-Barre syndrome (GBS) and chronic inflammatory demyelinating polyneuropathy (CIDP) towards Schwann cells using immunofluorescence microscopy. We found 24% of the GBS (56 out of 233) and 26% of the CIDP (12 out of 46) patients to have circulating immunoglobulin G autoantibodies against proliferating, non-myelinating human Schwann cells. In contrast, healthy donors showed positive staining in only two out of 34 sera. No reaction was found with sera from patients with non-inflammatory neurological disorders. Immunofluorescence was localized at the distal tips (leading lamella) of the Schwann cell processes. Distal tips of neurites (nerve-growth-cones) of in vitro differentiated non-myelinated hNT2 neurons also stained strongly. GBS and CIDP serum immunoreactivity was also observed in teased nerve fibre preparations. These data suggest that, at least part of the immunoreactivity is not directed against myelin, but towards non-myelin proteins and epitopes possibly involved in Schwann cell-axon interaction.

U2 - 10.1093/brain/awg030

DO - 10.1093/brain/awg030

M3 - Article

VL - 126

SP - 361

EP - 375

JO - Brain: a journal of neurology

JF - Brain: a journal of neurology

SN - 0006-8950

IS - 2

ER -

Kwa MSG, Van Schaik IN, Dejonge RR, Brand A, Kalaydjieva L, Van Belzen N et al. Autoimmunoreactivity to Schwann cells in patients with inflammatory neuropathies. Brain. 2003;126(2):361-375. https://doi.org/10.1093/brain/awg030