Assessment of serum total 25-hydroxyvitamin D assay commutability of Standard Reference Materials and College of American Pathologists Accuracy-Based Vitamin D (ABVD) Scheme and Vitamin D External Quality Assessment Scheme (DEQAS) materials: Vitamin D Standardization Program (VDSP) Commutability Study 2

Johanna E Camara, Stephen A Wise, Andrew N Hoofnagle, Emma L Williams, Graham D Carter, Julia Jones, Carolyn Q Burdette, Grace Hahm, Federica Nalin, Adam J Kuszak, Joyce Merkel, Ramón A Durazo-Arvizu, Pierre Lukas, Étienne Cavalier, Christian Popp, Christian Beckert, Jan Schultess, Glen Van Slooten, Carole Tourneur, Camille PeaseRavi Kaul, Alfredo Villarreal, Fiona Ivison, Ralf Fischer, Jody M W van den Ouweland, Chung S Ho, Emmett W K Law, Jean-Nicolas Simard, Renaud Gonthier, Brett Holmquist, Marcelo Cidade Batista, Heather Pham, Alex Bennett, Sarah Meadows, Lorna Cox, Eugene Jansen, Dilshad Ahmed Khan, Kimberly Robyak, Michael H Creer, Mark Kilbane, Patrick J Twomey, James Freeman, Neil Parker, Jinyun Yuan, Robert Fitzgerald, Sohail Mushtaq, Michael W Clarke, Norma Breen, Christine Simpson, Christopher T Sempos

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

An interlaboratory study was conducted through the Vitamin D Standardization Program (VDSP) to assess commutability of Standard Reference Materials® (SRMs) and proficiency testing/external quality assessment (PT/EQA) samples for determination of serum total 25-hydroxyvitamin D [25(OH)D] using ligand binding assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). A set of 50 single-donor serum samples were assigned target values for 25-hydroxyvitamin D2 [25(OH)D2] and 25-hydroxyvitamin D3 [25(OH)D3] using reference measurement procedures (RMPs). SRM and PT/EQA samples evaluated included SRM 972a (four levels), SRM 2973, six College of American Pathologists (CAP) Accuracy-Based Vitamin D (ABVD) samples, and nine Vitamin D External Quality Assessment Scheme (DEQAS) samples. Results were received from 28 different laboratories using 20 ligand binding assays and 14 LC-MS/MS methods. Using the test assay results for total serum 25(OH)D (i.e., the sum of 25(OH)D2 and 25(OH)D3) determined for the single-donor samples and the RMP target values, the linear regression and 95% prediction intervals (PIs) were calculated. Using a subset of 42 samples that had concentrations of 25(OH)D2 below 30 nmol/L, one or more of the SRM and PT/EQA samples with high concentrations of 25(OH)D2 were deemed non-commutable using 5 of 11 unique ligand binding assays. SRM 972a (level 4), which has high exogenous concentration of 3-epi-25(OH)D3, was deemed non-commutable for 50% of the LC-MS/MS assays.

Original languageEnglish
Pages (from-to)5067-5084
Number of pages18
JournalAnalytical and Bioanalytical Chemistry
Volume413
Issue number20
Early online date28 Jun 2021
DOIs
Publication statusPublished - Aug 2021

Fingerprint

Dive into the research topics of 'Assessment of serum total 25-hydroxyvitamin D assay commutability of Standard Reference Materials and College of American Pathologists Accuracy-Based Vitamin D (ABVD) Scheme and Vitamin D External Quality Assessment Scheme (DEQAS) materials: Vitamin D Standardization Program (VDSP) Commutability Study 2'. Together they form a unique fingerprint.

Cite this