Assessment of neutrophil chemotaxis by laser video densitometry

N.L.A. Misso, T. Kang, M.J. Phillips, Philip Thompson

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    Abstract

    A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.
    Original languageEnglish
    Pages (from-to)183-187
    JournalJournal of Immunological Methods
    Volume149
    DOIs
    Publication statusPublished - 1992

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