Assessment of neutrophil chemotaxis by laser video densitometry

N.L.A. Misso, T. Kang, M.J. Phillips, Philip Thompson

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Web of Science)


    A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.
    Original languageEnglish
    Pages (from-to)183-187
    JournalJournal of Immunological Methods
    Publication statusPublished - 1992


    Dive into the research topics of 'Assessment of neutrophil chemotaxis by laser video densitometry'. Together they form a unique fingerprint.

    Cite this