Assessment of neutrophil chemotaxis by laser video densitometry

N.L.A. Misso; T. Kang; M.J. Phillips; Philip Thompson

doi:10.1016/0022-1759(92)90249-S

Assessment of neutrophil chemotaxis by laser video densitometry

N.L.A. Misso, T. Kang, M.J. Phillips, Philip Thompson

Research output: Contribution to journal › Article › peer-review

3 Citations (Web of Science)

Abstract

A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.

Original language	English
Pages (from-to)	183-187
Journal	Journal of Immunological Methods
Volume	149
DOIs	https://doi.org/10.1016/0022-1759(92)90249-S
Publication status	Published - 1992

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title = "Assessment of neutrophil chemotaxis by laser video densitometry",

abstract = "A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.",

author = "N.L.A. Misso and T. Kang and M.J. Phillips and Philip Thompson",

year = "1992",

doi = "10.1016/0022-1759(92)90249-S",

language = "English",

volume = "149",

pages = "183--187",

journal = "Journal of Immunological Methods",

publisher = "Elsevier",

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T1 - Assessment of neutrophil chemotaxis by laser video densitometry

AU - Misso, N.L.A.

AU - Kang, T.

AU - Phillips, M.J.

AU - Thompson, Philip

PY - 1992

Y1 - 1992

N2 - A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.

AB - A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.

U2 - 10.1016/0022-1759(92)90249-S

DO - 10.1016/0022-1759(92)90249-S

M3 - Article

VL - 149

SP - 183

EP - 187

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

ER -

Assessment of neutrophil chemotaxis by laser video densitometry

Abstract

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