A laser densitometer and a video densitometer were used to evaluate neutrophil chemotaxis slides which were also assessed by the standard microscopic technique. A linear relationship was observed between cell number per high power field (HPF) and peak height by laser densitometry (r2 = 0.66) or peak area by video densitometry (r2 = 0.81). For wells containing more than 20 cells/HPF the least variability was observed with video densitometry. Quantitative results from neutrophil chemotaxis assays performed in 48-well microchambers can be obtained rapidly and conveniently by the use of commercially available video densitometers.
Misso, N. L. A., Kang, T., Phillips, M. J., & Thompson, P. (1992). Assessment of neutrophil chemotaxis by laser video densitometry. Journal of Immunological Methods, 149, 183-187. https://doi.org/10.1016/0022-1759(92)90249-S