Murine proteins of iron homoeostasis are frequently manipulated to investigate the mechanisms of iron-distribution and their toxicological consequences. Beyond subtracting erythrocyte-bound 59Fe of the residual blood content determined for each tissue (subtraction method), procedures are needed to determine 59Fe distribution in murine models of, e.g. inflammation or diabetes that cause local hyperaemia and changes in microcirculation.
Szegner, B., Herbach, N., Ettle, T., Elsenhans, B., & Schumann, K. (2008). Assessing and influencing the fractional contribution of erythrocyte-bound 59Fe to individual 59Fe tissue content in murine 59Fe distribution studies. Toxicology, 244(2-3), 198-208. https://doi.org/10.1016/j.tox.2007.11.015