Assay for urinary desmosines in a healthy pre-pubertal population using an improved extraction technique

K.R. Winfield, S. Gard, G.N. Kent, Peter Sly, S. Brennan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background Current evidence indicates that increased desmosine excretion reflects the active inflammatory status of some connective tissue diseases. Our goal was to establish a reliable method of detection and to investigate the normal distribution of urinary desmosine excretion in a healthy pre-pubertal population.Method Urine was collected from healthy volunteers aged four weeks to 12 years old. We modified a published high-performance liquid chromatography (HPLC) method by (a) increasing hydrolysis time and temperature and (b) increasing cellulose column size.Results Our modified method had small inter- and intra-assay variability, with coefficients of variation of <6.4% and 5.3%, respectively. There was positive correlation between isodesmosine and desmosine (r(2) = 0.91). There was no significant diurnal or day-to-day variability in total desmosine levels. A reference range for healthy pre-pubertal children aged four weeks to 12 years was established.Conclusion The modified HPLC method is reliable with low variability. The technique can now be applied as a non-invasive research or diagnostic tool for children with chronic lung disease.
Original languageEnglish
Pages (from-to)146-152
JournalAnnals of Clinical Biochemistry
Volume43
Issue number2
DOIs
Publication statusPublished - 2006

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