Aspergillus hancockii sp. nov., a biosynthetically talented fungus endemic to southeastern Australian soils

John I. Pitt, Lene Lange, Alastair E. Lacey, Daniel Vuong, David J. Midgley, Paul Greenfield, Mark I. Bradbury, Ernest Lacey, Peter K. Busk, Bo Pilgaard, Yit Heng Chooi, Andrew M. Piggott

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aspergillus hancockii sp. nov., classified in Aspergillus subgenus Circumdati section Flavi, was originally isolated from soil in peanut fields near Kumbia, in the South Burnett region of southeast Queensland, Australia, and has since been found occasionally from other substrates and locations in southeast Australia. It is phylogenetically and phenotypically related most closely to A. leporis States and M. Chr., but differs in conidial colour, other minor features and particularly in metabolite profile. When cultivated on rice as an optimal substrate, A. hancockii produced an extensive array of 69 secondary metabolites. Eleven of the 15 most abundant secondary metabolites, constituting 90% of the total area under the curve of the HPLC trace of the crude extract, were novel. The genome of A. hancockii, approximately 40 Mbp, was sequenced and mined for genes encoding carbohydrate degrading enzymes identified the presence of more than 370 genes in 114 gene clusters, demonstrating that A. hancockii has the capacity to degrade cellulose, hemicellulose, lignin, pectin, starch, chitin, cutin and fructan as nutrient sources. Like most Aspergillus species, A. hancockii exhibited a diverse secondary metabolite gene profile, encoding 26 polyketide synthase, 16 nonribosomal peptide synthase and 15 nonribosomal peptide synthase-like enzymes.

Original languageEnglish
Article numbere0170254
Number of pages21
JournalPLoS One
Volume12
Issue number4
DOIs
Publication statusPublished - 5 Apr 2017

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Aspergillus
Metabolites
Fungi
secondary metabolites
Soil
Soils
fungi
Gene encoding
Genes
new species
Fructans
peptides
Polyketide Synthases
polyketide synthases
cutin
soil
Queensland
Chitin
fructans
genes

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Pitt, J. I., Lange, L., Lacey, A. E., Vuong, D., Midgley, D. J., Greenfield, P., ... Piggott, A. M. (2017). Aspergillus hancockii sp. nov., a biosynthetically talented fungus endemic to southeastern Australian soils. PLoS One, 12(4), [e0170254]. https://doi.org/10.1371/journal.pone.0170254
Pitt, John I. ; Lange, Lene ; Lacey, Alastair E. ; Vuong, Daniel ; Midgley, David J. ; Greenfield, Paul ; Bradbury, Mark I. ; Lacey, Ernest ; Busk, Peter K. ; Pilgaard, Bo ; Chooi, Yit Heng ; Piggott, Andrew M. / Aspergillus hancockii sp. nov., a biosynthetically talented fungus endemic to southeastern Australian soils. In: PLoS One. 2017 ; Vol. 12, No. 4.
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Pitt, JI, Lange, L, Lacey, AE, Vuong, D, Midgley, DJ, Greenfield, P, Bradbury, MI, Lacey, E, Busk, PK, Pilgaard, B, Chooi, YH & Piggott, AM 2017, 'Aspergillus hancockii sp. nov., a biosynthetically talented fungus endemic to southeastern Australian soils' PLoS One, vol. 12, no. 4, e0170254. https://doi.org/10.1371/journal.pone.0170254

Aspergillus hancockii sp. nov., a biosynthetically talented fungus endemic to southeastern Australian soils. / Pitt, John I.; Lange, Lene; Lacey, Alastair E.; Vuong, Daniel; Midgley, David J.; Greenfield, Paul; Bradbury, Mark I.; Lacey, Ernest; Busk, Peter K.; Pilgaard, Bo; Chooi, Yit Heng; Piggott, Andrew M.

In: PLoS One, Vol. 12, No. 4, e0170254, 05.04.2017.

Research output: Contribution to journalArticle

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AU - Pitt, John I.

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