Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization

Spurthi N. Nayak, Gaurav Agarwal, Manish K. Pandey, Hari K. Sudini, Ashwin S. Jayale, Shilp Purohit, Aarthi Desai, Liyun Wan, Baozhu Guo, Boshou Liao, Rajeev K. Varshney

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaënsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.

Original languageEnglish
Article number9659
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Aspergillus flavus
Seeds
Infection
Genes
Aflatoxins
Host-Pathogen Interactions
Lipoxygenase
Growth and Development
Libraries
Breeding
In Vitro Techniques
Proteins
Fungi
Genotype
Genome
RNA
Health

Cite this

Nayak, Spurthi N. ; Agarwal, Gaurav ; Pandey, Manish K. ; Sudini, Hari K. ; Jayale, Ashwin S. ; Purohit, Shilp ; Desai, Aarthi ; Wan, Liyun ; Guo, Baozhu ; Liao, Boshou ; Varshney, Rajeev K. / Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{2fb86ee27cbe405fb419f2dd44f153ae,
title = "Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization",
abstract = "Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64{\%} and 67{\%} of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipa{\"e}nsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.",
author = "Nayak, {Spurthi N.} and Gaurav Agarwal and Pandey, {Manish K.} and Sudini, {Hari K.} and Jayale, {Ashwin S.} and Shilp Purohit and Aarthi Desai and Liyun Wan and Baozhu Guo and Boshou Liao and Varshney, {Rajeev K.}",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-09260-8",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group - Macmillan Publishers",
number = "1",

}

Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization. / Nayak, Spurthi N.; Agarwal, Gaurav; Pandey, Manish K.; Sudini, Hari K.; Jayale, Ashwin S.; Purohit, Shilp; Desai, Aarthi; Wan, Liyun; Guo, Baozhu; Liao, Boshou; Varshney, Rajeev K.

In: Scientific Reports, Vol. 7, No. 1, 9659, 01.12.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization

AU - Nayak, Spurthi N.

AU - Agarwal, Gaurav

AU - Pandey, Manish K.

AU - Sudini, Hari K.

AU - Jayale, Ashwin S.

AU - Purohit, Shilp

AU - Desai, Aarthi

AU - Wan, Liyun

AU - Guo, Baozhu

AU - Liao, Boshou

AU - Varshney, Rajeev K.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaënsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.

AB - Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaënsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.

UR - http://www.scopus.com/inward/record.url?scp=85028469881&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-09260-8

DO - 10.1038/s41598-017-09260-8

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 9659

ER -