Artifact-free high-density localization microscopy analysis

Richard J. Marsh, Karin Pfisterer, Pauline Bennett, Liisa M. Hirvonen, Mathias Gautel, Gareth E. Jones, Susan Cox

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

Original languageEnglish
Pages (from-to)689-692
Number of pages4
JournalNature Methods
Issue number9
Publication statusPublished - 1 Sep 2018
Externally publishedYes


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