Artifact-free high-density localization microscopy analysis

Richard J. Marsh, Karin Pfisterer, Pauline Bennett, Liisa M. Hirvonen, Mathias Gautel, Gareth E. Jones, Susan Cox

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

Original languageEnglish
Pages (from-to)689-692
Number of pages4
JournalNature Methods
Volume15
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018
Externally publishedYes

Fingerprint Dive into the research topics of 'Artifact-free high-density localization microscopy analysis'. Together they form a unique fingerprint.

  • Cite this

    Marsh, R. J., Pfisterer, K., Bennett, P., Hirvonen, L. M., Gautel, M., Jones, G. E., & Cox, S. (2018). Artifact-free high-density localization microscopy analysis. Nature Methods, 15(9), 689-692. https://doi.org/10.1038/s41592-018-0072-5