Arsenic uptake, translocation and speciation in pho 1 and pho 2 mutants of Arabidopsis thaliana

M. Quaghebeur, Zed Rengel

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Arsenate [As (V)] is taken up by phosphate [P (V)] transporters in the plasma membrane of roots cells, but the translocation of As from roots to shoots is not well understood. Two mutants of Arabidopsis thaliana (L.) [(pho1, P deficient) and (pho2, P accumulator)], with defects in the regulation and translocation of P (V) from roots to shoots, were therefore used in this study to investigate uptake, translocation and speciation of As in roots and shoots of plants grown in soil or nutrient solution. The shoots of the pho2 mutant contained higher P concentrations, but similar or slightly higher As concentrations, in comparison with the wild type. In the pho1 mutant, the P concentration in the shoots was lower, and the As concentration was higher, in comparison with the wild type. Both pho2 and the wild type contained mainly As (III) in roots and shoot (67–90% of total As). Arsenic was likely to be translocated by a different pathway to P (V) in the pho2 and pho1 mutants. Therefore, it is suggested that As (III) is the main As species translocated from roots to shoots in Arabidopsis thaliana.
Original languageEnglish
Pages (from-to)280-286
JournalPhysiologia Plantarum
Volume120
Issue number2
DOIs
Publication statusPublished - 2004

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Arsenic
arsenic
Arabidopsis
Arabidopsis thaliana
Plant Shoots
mutants
Plant Roots
shoots
Soil
Phosphates
Cell Membrane
arsenates
soil solution
nutrient solutions
transporters
plasma membrane
phosphates
arsenic acid

Cite this

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title = "Arsenic uptake, translocation and speciation in pho 1 and pho 2 mutants of Arabidopsis thaliana",
abstract = "Arsenate [As (V)] is taken up by phosphate [P (V)] transporters in the plasma membrane of roots cells, but the translocation of As from roots to shoots is not well understood. Two mutants of Arabidopsis thaliana (L.) [(pho1, P deficient) and (pho2, P accumulator)], with defects in the regulation and translocation of P (V) from roots to shoots, were therefore used in this study to investigate uptake, translocation and speciation of As in roots and shoots of plants grown in soil or nutrient solution. The shoots of the pho2 mutant contained higher P concentrations, but similar or slightly higher As concentrations, in comparison with the wild type. In the pho1 mutant, the P concentration in the shoots was lower, and the As concentration was higher, in comparison with the wild type. Both pho2 and the wild type contained mainly As (III) in roots and shoot (67–90{\%} of total As). Arsenic was likely to be translocated by a different pathway to P (V) in the pho2 and pho1 mutants. Therefore, it is suggested that As (III) is the main As species translocated from roots to shoots in Arabidopsis thaliana.",
author = "M. Quaghebeur and Zed Rengel",
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volume = "120",
pages = "280--286",
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Arsenic uptake, translocation and speciation in pho 1 and pho 2 mutants of Arabidopsis thaliana. / Quaghebeur, M.; Rengel, Zed.

In: Physiologia Plantarum, Vol. 120, No. 2, 2004, p. 280-286.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Arsenic uptake, translocation and speciation in pho 1 and pho 2 mutants of Arabidopsis thaliana

AU - Quaghebeur, M.

AU - Rengel, Zed

PY - 2004

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AB - Arsenate [As (V)] is taken up by phosphate [P (V)] transporters in the plasma membrane of roots cells, but the translocation of As from roots to shoots is not well understood. Two mutants of Arabidopsis thaliana (L.) [(pho1, P deficient) and (pho2, P accumulator)], with defects in the regulation and translocation of P (V) from roots to shoots, were therefore used in this study to investigate uptake, translocation and speciation of As in roots and shoots of plants grown in soil or nutrient solution. The shoots of the pho2 mutant contained higher P concentrations, but similar or slightly higher As concentrations, in comparison with the wild type. In the pho1 mutant, the P concentration in the shoots was lower, and the As concentration was higher, in comparison with the wild type. Both pho2 and the wild type contained mainly As (III) in roots and shoot (67–90% of total As). Arsenic was likely to be translocated by a different pathway to P (V) in the pho2 and pho1 mutants. Therefore, it is suggested that As (III) is the main As species translocated from roots to shoots in Arabidopsis thaliana.

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DO - 10.1111/j.0031-9317.2004.0240.x

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