Arcuate nucleus kisspeptin response to increased nutrition in rams

S. E. Rietema, P. A.R. Hawken, C. J. Scott, M. N. Lehman, G. B. Martin, J. T. Smith

Research output: Contribution to journalArticle

79 Downloads (Pure)

Abstract

Rams respond to acute nutritional supplementation by increasing the frequency of gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the effect of environmental cues on GnRH secretion, so we tested whether the ram response to nutrition involves activation of kisspeptin neurons in the arcuate nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were given extra lupin grain with their normal ration. Blood was sampled before feeding, and continued until animals were killed for collection of brain tissue at 2 or 11 h after supplementation. In supplemented rams, LH pulse frequency increased after feeding, whereas control animals showed no change. Within the caudal ARC, there were more kisspeptin neurons in supplemented rams than in controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless of the time the rams were killed. There were more Fos cells in the mid-ARC and mid-dorsomedial hypothalamus of the supplemented compared with control rams. No effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC appear to mediate the increase in GnRH and LH production due to acute nutritional supplementation, supporting the hypothesised role of the KNDy neurons as the pulse generator for GnRH.

Original languageEnglish
Pages (from-to)1682-1691
Number of pages10
JournalReproduction, Fertility and Development
Volume31
Issue number11
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Kisspeptins
Arcuate Nucleus of Hypothalamus
rams
gonadotropin-releasing hormone
nutrition
neurons
Gonadotropin-Releasing Hormone
Neurons
legumes
preoptic area
Lupinus
hormone secretion
Preoptic Area
hypothalamus
animals
Hypothalamus
Cues
cells
brain
blood

Cite this

Rietema, S. E., Hawken, P. A. R., Scott, C. J., Lehman, M. N., Martin, G. B., & Smith, J. T. (2019). Arcuate nucleus kisspeptin response to increased nutrition in rams. Reproduction, Fertility and Development, 31(11), 1682-1691. https://doi.org/10.1071/RD19063
Rietema, S. E. ; Hawken, P. A.R. ; Scott, C. J. ; Lehman, M. N. ; Martin, G. B. ; Smith, J. T. / Arcuate nucleus kisspeptin response to increased nutrition in rams. In: Reproduction, Fertility and Development. 2019 ; Vol. 31, No. 11. pp. 1682-1691.
@article{46147d044a6e4f9d9f5507e30a702745,
title = "Arcuate nucleus kisspeptin response to increased nutrition in rams",
abstract = "Rams respond to acute nutritional supplementation by increasing the frequency of gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the effect of environmental cues on GnRH secretion, so we tested whether the ram response to nutrition involves activation of kisspeptin neurons in the arcuate nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were given extra lupin grain with their normal ration. Blood was sampled before feeding, and continued until animals were killed for collection of brain tissue at 2 or 11 h after supplementation. In supplemented rams, LH pulse frequency increased after feeding, whereas control animals showed no change. Within the caudal ARC, there were more kisspeptin neurons in supplemented rams than in controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless of the time the rams were killed. There were more Fos cells in the mid-ARC and mid-dorsomedial hypothalamus of the supplemented compared with control rams. No effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC appear to mediate the increase in GnRH and LH production due to acute nutritional supplementation, supporting the hypothesised role of the KNDy neurons as the pulse generator for GnRH.",
keywords = "energy balance, fertility, neuroendocrinology, reproduction",
author = "Rietema, {S. E.} and Hawken, {P. A.R.} and Scott, {C. J.} and Lehman, {M. N.} and Martin, {G. B.} and Smith, {J. T.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1071/RD19063",
language = "English",
volume = "31",
pages = "1682--1691",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO Publishing",
number = "11",

}

Rietema, SE, Hawken, PAR, Scott, CJ, Lehman, MN, Martin, GB & Smith, JT 2019, 'Arcuate nucleus kisspeptin response to increased nutrition in rams' Reproduction, Fertility and Development, vol. 31, no. 11, pp. 1682-1691. https://doi.org/10.1071/RD19063

Arcuate nucleus kisspeptin response to increased nutrition in rams. / Rietema, S. E.; Hawken, P. A.R.; Scott, C. J.; Lehman, M. N.; Martin, G. B.; Smith, J. T.

In: Reproduction, Fertility and Development, Vol. 31, No. 11, 01.01.2019, p. 1682-1691.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Arcuate nucleus kisspeptin response to increased nutrition in rams

AU - Rietema, S. E.

AU - Hawken, P. A.R.

AU - Scott, C. J.

AU - Lehman, M. N.

AU - Martin, G. B.

AU - Smith, J. T.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Rams respond to acute nutritional supplementation by increasing the frequency of gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the effect of environmental cues on GnRH secretion, so we tested whether the ram response to nutrition involves activation of kisspeptin neurons in the arcuate nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were given extra lupin grain with their normal ration. Blood was sampled before feeding, and continued until animals were killed for collection of brain tissue at 2 or 11 h after supplementation. In supplemented rams, LH pulse frequency increased after feeding, whereas control animals showed no change. Within the caudal ARC, there were more kisspeptin neurons in supplemented rams than in controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless of the time the rams were killed. There were more Fos cells in the mid-ARC and mid-dorsomedial hypothalamus of the supplemented compared with control rams. No effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC appear to mediate the increase in GnRH and LH production due to acute nutritional supplementation, supporting the hypothesised role of the KNDy neurons as the pulse generator for GnRH.

AB - Rams respond to acute nutritional supplementation by increasing the frequency of gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the effect of environmental cues on GnRH secretion, so we tested whether the ram response to nutrition involves activation of kisspeptin neurons in the arcuate nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were given extra lupin grain with their normal ration. Blood was sampled before feeding, and continued until animals were killed for collection of brain tissue at 2 or 11 h after supplementation. In supplemented rams, LH pulse frequency increased after feeding, whereas control animals showed no change. Within the caudal ARC, there were more kisspeptin neurons in supplemented rams than in controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless of the time the rams were killed. There were more Fos cells in the mid-ARC and mid-dorsomedial hypothalamus of the supplemented compared with control rams. No effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC appear to mediate the increase in GnRH and LH production due to acute nutritional supplementation, supporting the hypothesised role of the KNDy neurons as the pulse generator for GnRH.

KW - energy balance

KW - fertility

KW - neuroendocrinology

KW - reproduction

UR - http://www.scopus.com/inward/record.url?scp=85072257150&partnerID=8YFLogxK

U2 - 10.1071/RD19063

DO - 10.1071/RD19063

M3 - Article

VL - 31

SP - 1682

EP - 1691

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 11

ER -