Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea

Abeer Hashem, Ashwani Kumar, Abeer M. Al-Dbass, Abdulaziz A. Alqarawi, Al-Bandari Fahad Al-Arjani, Garima Singh, Muhammad Farooq, Elsayed Fathi Abd Allah

Research output: Contribution to journalArticle

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Abstract

Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the performance of chickpea under drought stress. The treatments included the individual as well as combined treatment of AMF and biochar to drought stressed and normal chickpea plants. Plants inoculation improved growth in terms of shoot and root length, leaf area and number of branches which was observed to show a steep decline due to drought stress. Drought declined the AMF colonization potential though biochar amendment ameliorated the negative effects of drought significantly by improving the spore population, number of mycelium, vesicle and arbuscules and the percentage of colonization as well. Increased chlorophyll synthesis in biochar and AMF treated plants was obvious, which lead to significant enhancement in the net photosynthetic efficiency. Drought stress also declined the relative water content (RWC) and membrane stability index (MSI), while treatment of biochar and AMF either individually or in combination mitigated the deleterious effects to considerable extent and caused a significant enhancement in RWC and MSI under normal conditions. Amendments with biochar and AMF inoculation increased the nitrogen fixation attributes including the number and weight of nodules, leghe-moglobin content and activity of nitrate reductase enzyme leading to greater uptake and assimilation of nitrogen in them when compared to drought stressed plants. Drought stressed chickpea plants exhibited considerable reduction in uptake of nitrogen and phosphorous which was ameliorated by biochar and AMF treatments. It could be suggested that increase in growth and physiological attributes in chickpea due to biochar amendments and AMF inoculation under drought stress were plausibly due to their involvement in nitrogen and phosphorous uptake, chlorophyll synthesis and photosynthesis. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.

Original languageEnglish
Pages (from-to)614-624
Number of pages11
JournalSaudi Journal of Biological Sciences
Volume26
Issue number3
DOIs
Publication statusPublished - Mar 2019

Cite this

Hashem, A., Kumar, A., Al-Dbass, A. M., Alqarawi, A. A., Al-Arjani, A-B. F., Singh, G., ... Abd Allah, E. F. (2019). Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea. Saudi Journal of Biological Sciences, 26(3), 614-624. https://doi.org/10.1016/j.sjbs.2018.11.005
Hashem, Abeer ; Kumar, Ashwani ; Al-Dbass, Abeer M. ; Alqarawi, Abdulaziz A. ; Al-Arjani, Al-Bandari Fahad ; Singh, Garima ; Farooq, Muhammad ; Abd Allah, Elsayed Fathi. / Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea. In: Saudi Journal of Biological Sciences. 2019 ; Vol. 26, No. 3. pp. 614-624.
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abstract = "Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the performance of chickpea under drought stress. The treatments included the individual as well as combined treatment of AMF and biochar to drought stressed and normal chickpea plants. Plants inoculation improved growth in terms of shoot and root length, leaf area and number of branches which was observed to show a steep decline due to drought stress. Drought declined the AMF colonization potential though biochar amendment ameliorated the negative effects of drought significantly by improving the spore population, number of mycelium, vesicle and arbuscules and the percentage of colonization as well. Increased chlorophyll synthesis in biochar and AMF treated plants was obvious, which lead to significant enhancement in the net photosynthetic efficiency. Drought stress also declined the relative water content (RWC) and membrane stability index (MSI), while treatment of biochar and AMF either individually or in combination mitigated the deleterious effects to considerable extent and caused a significant enhancement in RWC and MSI under normal conditions. Amendments with biochar and AMF inoculation increased the nitrogen fixation attributes including the number and weight of nodules, leghe-moglobin content and activity of nitrate reductase enzyme leading to greater uptake and assimilation of nitrogen in them when compared to drought stressed plants. Drought stressed chickpea plants exhibited considerable reduction in uptake of nitrogen and phosphorous which was ameliorated by biochar and AMF treatments. It could be suggested that increase in growth and physiological attributes in chickpea due to biochar amendments and AMF inoculation under drought stress were plausibly due to their involvement in nitrogen and phosphorous uptake, chlorophyll synthesis and photosynthesis. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.",
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Hashem, A, Kumar, A, Al-Dbass, AM, Alqarawi, AA, Al-Arjani, A-BF, Singh, G, Farooq, M & Abd Allah, EF 2019, 'Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea' Saudi Journal of Biological Sciences, vol. 26, no. 3, pp. 614-624. https://doi.org/10.1016/j.sjbs.2018.11.005

Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea. / Hashem, Abeer; Kumar, Ashwani; Al-Dbass, Abeer M.; Alqarawi, Abdulaziz A.; Al-Arjani, Al-Bandari Fahad; Singh, Garima; Farooq, Muhammad; Abd Allah, Elsayed Fathi.

In: Saudi Journal of Biological Sciences, Vol. 26, No. 3, 03.2019, p. 614-624.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Arbuscular mycorrhizal fungi and biochar improves drought tolerance in chickpea

AU - Hashem, Abeer

AU - Kumar, Ashwani

AU - Al-Dbass, Abeer M.

AU - Alqarawi, Abdulaziz A.

AU - Al-Arjani, Al-Bandari Fahad

AU - Singh, Garima

AU - Farooq, Muhammad

AU - Abd Allah, Elsayed Fathi

PY - 2019/3

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N2 - Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the performance of chickpea under drought stress. The treatments included the individual as well as combined treatment of AMF and biochar to drought stressed and normal chickpea plants. Plants inoculation improved growth in terms of shoot and root length, leaf area and number of branches which was observed to show a steep decline due to drought stress. Drought declined the AMF colonization potential though biochar amendment ameliorated the negative effects of drought significantly by improving the spore population, number of mycelium, vesicle and arbuscules and the percentage of colonization as well. Increased chlorophyll synthesis in biochar and AMF treated plants was obvious, which lead to significant enhancement in the net photosynthetic efficiency. Drought stress also declined the relative water content (RWC) and membrane stability index (MSI), while treatment of biochar and AMF either individually or in combination mitigated the deleterious effects to considerable extent and caused a significant enhancement in RWC and MSI under normal conditions. Amendments with biochar and AMF inoculation increased the nitrogen fixation attributes including the number and weight of nodules, leghe-moglobin content and activity of nitrate reductase enzyme leading to greater uptake and assimilation of nitrogen in them when compared to drought stressed plants. Drought stressed chickpea plants exhibited considerable reduction in uptake of nitrogen and phosphorous which was ameliorated by biochar and AMF treatments. It could be suggested that increase in growth and physiological attributes in chickpea due to biochar amendments and AMF inoculation under drought stress were plausibly due to their involvement in nitrogen and phosphorous uptake, chlorophyll synthesis and photosynthesis. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.

AB - Arbuscular mycorrhizal fungi (AMF) inoculation and biochar amendment has been reported to improve growth of several crop plants however their role in stress amelioration individually as well as in combination has not been worked out. This experiment was conducted to evaluate the application of AMF and biochar on the performance of chickpea under drought stress. The treatments included the individual as well as combined treatment of AMF and biochar to drought stressed and normal chickpea plants. Plants inoculation improved growth in terms of shoot and root length, leaf area and number of branches which was observed to show a steep decline due to drought stress. Drought declined the AMF colonization potential though biochar amendment ameliorated the negative effects of drought significantly by improving the spore population, number of mycelium, vesicle and arbuscules and the percentage of colonization as well. Increased chlorophyll synthesis in biochar and AMF treated plants was obvious, which lead to significant enhancement in the net photosynthetic efficiency. Drought stress also declined the relative water content (RWC) and membrane stability index (MSI), while treatment of biochar and AMF either individually or in combination mitigated the deleterious effects to considerable extent and caused a significant enhancement in RWC and MSI under normal conditions. Amendments with biochar and AMF inoculation increased the nitrogen fixation attributes including the number and weight of nodules, leghe-moglobin content and activity of nitrate reductase enzyme leading to greater uptake and assimilation of nitrogen in them when compared to drought stressed plants. Drought stressed chickpea plants exhibited considerable reduction in uptake of nitrogen and phosphorous which was ameliorated by biochar and AMF treatments. It could be suggested that increase in growth and physiological attributes in chickpea due to biochar amendments and AMF inoculation under drought stress were plausibly due to their involvement in nitrogen and phosphorous uptake, chlorophyll synthesis and photosynthesis. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.

KW - Biochar

KW - Arbuscular mycorrhizal fungi

KW - Membrane stability index

KW - Nitrogen fixation

KW - Photosynthesis

KW - Drought

KW - Cicer arietinum

KW - SALT STRESS

KW - PLANT-GROWTH

KW - PHOTOSYNTHESIS

KW - L.

KW - RESISTANCE

KW - INOCULATION

KW - ALLEVIATION

KW - ROOTS

KW - YIELD

KW - FERTILIZATION

U2 - 10.1016/j.sjbs.2018.11.005

DO - 10.1016/j.sjbs.2018.11.005

M3 - Article

VL - 26

SP - 614

EP - 624

JO - Saudi Journal of Biological Sciences

JF - Saudi Journal of Biological Sciences

SN - 1319-562X

IS - 3

ER -