Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities

B. De Coninck; K. Le Roy; I. Francis; S. Clerens; R. Vergauwen; A.M. Halliday; Steven Smith; A. Van Laere; W. Van Den Ende

doi:10.1111/j.1365-3040.2004.01281.x

Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities

B. De Coninck, K. Le Roy, I. Francis, S. Clerens, R. Vergauwen, A.M. Halliday, Steven Smith, A. Van Laere, W. Van Den Ende

School of Molecular Sciences

Research output: Contribution to journal › Article › peer-review

118 Citations (Scopus)

Abstract

The genome ofArabidopsis thalianacontains six putativecell-wall type invertase genes (AtcwINV1-6). Heterologousexpression ofAtcwINV1, 3and6cDNAs inPichiapastorisrevealed that the enzymes encoded byAtcwINV3and6did not show invertase activity. Instead, AtcwINV3is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH)that can degrade both inulin and levan-type fructans. ForAtcwINV6 it is proposed to use the term (6&1) FEH. Incontrast, AtcwINV1 is a typical invertase. FEH activity wasalso detected in crude extracts of different parts of Arabidopsis.To verify that the FEH activity ofAtcwINV3and6were not artefacts of the heterologous expression system,the protein corresponding to AtcwINV3 was isolated fromwhole Arabidopsis plants and indeed showed only 6-FEHactivity and no invertase activity. Although no fructans canbe detected in Arabidopsis plants, it is shown that kestoses(trimers) can be synthesized in crude leaf extracts. Theputative physiological significance of FEH in so-called nonfructanplants is discussed.

Original language	English
Pages (from-to)	432-443
Journal	Plant, Cell and Environment
Volume	28
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-3040.2004.01281.x
Publication status	Published - 2005

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@article{26a265fefaf64ea3973a6a34fa63a036,

title = "Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities",

abstract = "The genome ofArabidopsis thalianacontains six putativecell-wall type invertase genes (AtcwINV1-6). Heterologousexpression ofAtcwINV1, 3and6cDNAs inPichiapastorisrevealed that the enzymes encoded byAtcwINV3and6did not show invertase activity. Instead, AtcwINV3is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH)that can degrade both inulin and levan-type fructans. ForAtcwINV6 it is proposed to use the term (6&1) FEH. Incontrast, AtcwINV1 is a typical invertase. FEH activity wasalso detected in crude extracts of different parts of Arabidopsis.To verify that the FEH activity ofAtcwINV3and6were not artefacts of the heterologous expression system,the protein corresponding to AtcwINV3 was isolated fromwhole Arabidopsis plants and indeed showed only 6-FEHactivity and no invertase activity. Although no fructans canbe detected in Arabidopsis plants, it is shown that kestoses(trimers) can be synthesized in crude leaf extracts. Theputative physiological significance of FEH in so-called nonfructanplants is discussed.",

author = "{De Coninck}, B. and {Le Roy}, K. and I. Francis and S. Clerens and R. Vergauwen and A.M. Halliday and Steven Smith and {Van Laere}, A. and {Van Den Ende}, W.",

year = "2005",

doi = "10.1111/j.1365-3040.2004.01281.x",

language = "English",

volume = "28",

pages = "432--443",

journal = "Plant, Cell and Environment",

issn = "0140-7791",

publisher = "John Wiley & Sons",

number = "4",

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TY - JOUR

T1 - Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities

AU - De Coninck, B.

AU - Le Roy, K.

AU - Francis, I.

AU - Clerens, S.

AU - Vergauwen, R.

AU - Halliday, A.M.

AU - Smith, Steven

AU - Van Laere, A.

AU - Van Den Ende, W.

PY - 2005

Y1 - 2005

N2 - The genome ofArabidopsis thalianacontains six putativecell-wall type invertase genes (AtcwINV1-6). Heterologousexpression ofAtcwINV1, 3and6cDNAs inPichiapastorisrevealed that the enzymes encoded byAtcwINV3and6did not show invertase activity. Instead, AtcwINV3is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH)that can degrade both inulin and levan-type fructans. ForAtcwINV6 it is proposed to use the term (6&1) FEH. Incontrast, AtcwINV1 is a typical invertase. FEH activity wasalso detected in crude extracts of different parts of Arabidopsis.To verify that the FEH activity ofAtcwINV3and6were not artefacts of the heterologous expression system,the protein corresponding to AtcwINV3 was isolated fromwhole Arabidopsis plants and indeed showed only 6-FEHactivity and no invertase activity. Although no fructans canbe detected in Arabidopsis plants, it is shown that kestoses(trimers) can be synthesized in crude leaf extracts. Theputative physiological significance of FEH in so-called nonfructanplants is discussed.

AB - The genome ofArabidopsis thalianacontains six putativecell-wall type invertase genes (AtcwINV1-6). Heterologousexpression ofAtcwINV1, 3and6cDNAs inPichiapastorisrevealed that the enzymes encoded byAtcwINV3and6did not show invertase activity. Instead, AtcwINV3is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH)that can degrade both inulin and levan-type fructans. ForAtcwINV6 it is proposed to use the term (6&1) FEH. Incontrast, AtcwINV1 is a typical invertase. FEH activity wasalso detected in crude extracts of different parts of Arabidopsis.To verify that the FEH activity ofAtcwINV3and6were not artefacts of the heterologous expression system,the protein corresponding to AtcwINV3 was isolated fromwhole Arabidopsis plants and indeed showed only 6-FEHactivity and no invertase activity. Although no fructans canbe detected in Arabidopsis plants, it is shown that kestoses(trimers) can be synthesized in crude leaf extracts. Theputative physiological significance of FEH in so-called nonfructanplants is discussed.

U2 - 10.1111/j.1365-3040.2004.01281.x

DO - 10.1111/j.1365-3040.2004.01281.x

M3 - Article

SN - 0140-7791

VL - 28

SP - 432

EP - 443

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 4

ER -

Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities

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