Projects per year
Abstract
RNA binding proteins play integral roles in the regulation of essential processes in cells, and as such are attractive targets for engineering to manipulate gene expression at the RNA level. Expression of transcripts in chloroplasts and mitochondria is heavily regulated by pentatricopeptide repeat (PPR) proteins. The diverse roles of PPR proteins, and their naturally modular architecture, makes them ideal candidates for engineering. Synthetic PPR proteins are showing great potential to become valuable tools for controlling the expression of plastid and mitochondrial transcripts. In this review, by 'synthetic' we mean both rationally modified natural PPR proteins and completely novel proteins designed using the principles learnt from their natural counterparts. We focus on the many different applications of synthetic PPR proteins, covering both their use in basic research to learn more about protein-RNA interactions, and their use to achieve specific outcomes in RNA processing and the control of gene expression. We describe the challenges associated with the design, construction and deployment of synthetic PPR proteins and provide perspectives on how they might be assembled and used in future biotechnology applications.
Original language | English |
---|---|
Pages (from-to) | 503-515 |
Number of pages | 13 |
Journal | Plant & Cell Physiology |
Volume | 65 |
Issue number | 4 |
Early online date | 12 Dec 2023 |
DOIs | |
Publication status | Published - 1 Apr 2024 |
Fingerprint
Dive into the research topics of 'Applications of Synthetic Pentatricopeptide Repeat Proteins'. Together they form a unique fingerprint.Projects
- 3 Finished
-
IDENTIFYING CONTROL ELEMENTS IN CHLOROPLAST GENE EXPRESSION
Small, I. (Investigator 01)
ARC Australian Research Council
1/11/21 → 31/10/24
Project: Research
-
Mastering pyrimidine editing in RNA
Small, I. (Investigator 01) & Bond, C. (Investigator 02)
ARC Australian Research Council
1/01/20 → 31/12/24
Project: Research
-
ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H. (Investigator 01), Pogson, B. (Investigator 02), Tyerman, S. (Investigator 03), Small, I. (Investigator 04), Whelan, J. (Investigator 05), Borevitz, J. (Investigator 06), Lister, R. (Investigator 07), Atkin, O. (Investigator 08) & Munns, R. (Investigator 09)
ARC Australian Research Council
1/01/14 → 31/05/21
Project: Research