This study presents an unprecedented campaign of measurements (magnitudes and orientations) of the in situ stresses in itabirite rocks of the Brazilian Iron Quadrangle region using the hydraulic fracturing (HF), deformation rate analysis (DRA) and diametrical core deformation analysis (DCDA) methods. The previous studies of this rock mass consider estimated values of k, and the practice of using in situ stress tests to support geotechnical analyses of the rock mass of this region has not been adopted. The study site is located at a depth of up to 400 m around a pit. The rock mass under study shows the presence of different geological structures, which made performing the tests difficult. The DRA results show a marked difference compared to the HF and DCDA results; the horizontal stresses were greater than the vertical stresses, which is a finding that may occur due to local geodynamic influences. The results contribute to the understanding of the strains and stresses induced by mining activities in the Brazilian Iron Quadrangle. For a better determination of the regional in situ stresses in the rock mass of the Brazilian Iron Quadrangle, new HF tests, as well as hydraulic testing of pre-existing fractures are recommended.