Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart; Elizabeth Johnstone; A.J. Wheal; J. Goulding; M.B. Robers; T. Machleidt; K.V. Wood; Stephen Hill; Kevin Pfleger

doi:10.1038/nmeth.3398

Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart, Elizabeth Johnstone, A.J. Wheal, J. Goulding, M.B. Robers, T. Machleidt, K.V. Wood, Stephen Hill, Kevin Pfleger

Research output: Contribution to journal › Article › peer-review

176 Citations (Scopus)

Abstract

© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

Original language	English
Pages (from-to)	661-663
Number of pages	3
Journal	Nature Methods
Volume	12
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.3398
Publication status	Published - Jul 2015

Access to Document

10.1038/nmeth.3398

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488387/Licence: Other

Cite this

@article{85afcf652724438fa701c052e9d5733c,

title = "Application of BRET to monitor ligand binding to GPCRs",

abstract = "{\textcopyright} 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.",

author = "L.A. Stoddart and Elizabeth Johnstone and A.J. Wheal and J. Goulding and M.B. Robers and T. Machleidt and K.V. Wood and Stephen Hill and Kevin Pfleger",

year = "2015",

month = jul,

doi = "10.1038/nmeth.3398",

language = "English",

volume = "12",

pages = "661--663",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group - Macmillan Publishers",

number = "7",

}

TY - JOUR

T1 - Application of BRET to monitor ligand binding to GPCRs

AU - Stoddart, L.A.

AU - Johnstone, Elizabeth

AU - Wheal, A.J.

AU - Goulding, J.

AU - Robers, M.B.

AU - Machleidt, T.

AU - Wood, K.V.

AU - Hill, Stephen

AU - Pfleger, Kevin

PY - 2015/7

Y1 - 2015/7

N2 - © 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

AB - © 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

U2 - 10.1038/nmeth.3398

DO - 10.1038/nmeth.3398

M3 - Article

C2 - 26030448

VL - 12

SP - 661

EP - 663

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 7

ER -

Application of BRET to monitor ligand binding to GPCRs

Abstract

Access to Document

Fingerprint

Cite this