Abstract
© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Original language | English |
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Pages (from-to) | 661-663 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 12 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2015 |