Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart, Elizabeth Johnstone, A.J. Wheal, J. Goulding, M.B. Robers, T. Machleidt, K.V. Wood, Stephen Hill, Kevin Pfleger

Research output: Contribution to journalArticlepeer-review

176 Citations (Scopus)


© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Original languageEnglish
Pages (from-to)661-663
Number of pages3
JournalNature Methods
Issue number7
Publication statusPublished - Jul 2015


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