© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Stoddart, L. A., Johnstone, E., Wheal, A. J., Goulding, J., Robers, M. B., Machleidt, T., Wood, K. V., Hill, S., & Pfleger, K. (2015). Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12(7), 661-663. https://doi.org/10.1038/nmeth.3398