Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart, Elizabeth Johnstone, A.J. Wheal, J. Goulding, M.B. Robers, T. Machleidt, K.V. Wood, Stephen Hill, Kevin Pfleger

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100 Citations (Scopus)

Abstract

© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Original languageEnglish
Pages (from-to)661-663
Number of pages3
JournalNature Methods
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 2015

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    Stoddart, L. A., Johnstone, E., Wheal, A. J., Goulding, J., Robers, M. B., Machleidt, T., Wood, K. V., Hill, S., & Pfleger, K. (2015). Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12(7), 661-663. https://doi.org/10.1038/nmeth.3398