Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart; Elizabeth Johnstone; A.J. Wheal; J. Goulding; M.B. Robers; T. Machleidt; K.V. Wood; Stephen Hill; Kevin Pfleger

doi:10.1038/nmeth.3398

Application of BRET to monitor ligand binding to GPCRs

L.A. Stoddart, Elizabeth Johnstone, A.J. Wheal, J. Goulding, M.B. Robers, T. Machleidt, K.V. Wood, Stephen Hill, Kevin Pfleger

UWA Centre for Medical Research (affiliated with the Harry Perkins Institute of Medical Research)

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144 Citations (Scopus)

Abstract

© 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

Original language	English
Pages (from-to)	661-663
Number of pages	3
Journal	Nature Methods
Volume	12
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.3398
Publication status	Published - Jul 2015

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488387/Licence: Other

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abstract = "{\textcopyright} 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.",

author = "L.A. Stoddart and Elizabeth Johnstone and A.J. Wheal and J. Goulding and M.B. Robers and T. Machleidt and K.V. Wood and Stephen Hill and Kevin Pfleger",

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AU - Johnstone, Elizabeth

AU - Wheal, A.J.

AU - Goulding, J.

AU - Robers, M.B.

AU - Machleidt, T.

AU - Wood, K.V.

AU - Hill, Stephen

AU - Pfleger, Kevin

PY - 2015/7

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N2 - © 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

AB - © 2015 Nature America, Inc. All rights reserved. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.

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DO - 10.1038/nmeth.3398

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JO - Nature Methods

JF - Nature Methods

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Application of BRET to monitor ligand binding to GPCRs

Abstract

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