Application of 15N labeling to measure protein turnover rate in Arabidopsis thaliana

Lei Li

Research output: ThesisDoctoral Thesis

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Abstract

[Truncated abstract] Intracellular protein abundance changes are brought about by alteration in protein synthesis and/or degradation rates. Using a combination of 15N metabolic labeling, two-dimensional electrophoresis and MALDI-TOF/TOF mass spectrometry, a new method was developed to follow the progressive 15N labeling of Arabidopsis cells under non-steady conditions to calculate protein degradation rate (KD) and protein synthesis rate (KS). Over 65-fold variation in degradation rates was observed in 84 proteins across 6 different functional groups. Correlation between biological functional groups and protein turnover rates, protein degradation and synthesis rates, mRNA and protein turnover rates were discussed. An interesting question for protein turnover research is how macro molecular protein complexes containing both mitochondrial and nuclear encoded sybunits assembled together. Using the strategy developed here, subunits with F1 subcomplexes of ATP synthase in the mitochondrial inner membrane and matrix were found to have a relatively higher 15N incorporation rate than corresponding subunits in intact F1F0 ATP synthase...
Original languageEnglish
QualificationDoctor of Philosophy
Publication statusUnpublished - 2012

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