Appearance of the liver form of pyruvate kinase in differentiating cultured foetal hepatocytes

Rodney J. Scott, George C.T. Yeoh

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    From about the 16th day of gestation three forms of pyruvate kinase are present in foetal rat liver (L, R, and M2). Hepatocytes isolated from 15-day-old foetuses do not possess the liver form of pyruvate kinase, but after three days in culture this enzyme can be detected. No effect on the appearance of the enzyme could be seen by administration of insulin and fructose. Hepatocytes isolated from 19-day-old foetuses exhibit three forms of the enzyme (L, R, and M2) on day 1 of culture but thereafter only two forms are detectable (L and M2). A decrease in activity of the L form is observed. This could be retarded by administration of insulin and fructose.

    Original languageEnglish
    Pages (from-to)64-69
    Number of pages6
    JournalDifferentiation
    Volume25
    Issue number1-3
    DOIs
    Publication statusPublished - 1 Jan 1984

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