Apoptosis induction and reduced proliferation in human osteoblasts by rhBMP-2, -4 and -7

O. Gautschi, D. Cadosch, R. Zellweger, K.A. Joesbury, Luis Filgueira

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Background: The role of bone morphogenetic proteins (BMPs) in bone healing has been demonstrated in numerous in vivo animal models. BMP-2, -4 and -7 have also been shown to stimulate the differentiation of human and animal stem cells into osteoblasts in vitro. There are, however, contradictory reports of BMPs causing apoptosis and inhibition of proliferation of osteoblastic cells. Therefore, a more complete understanding of the effects of BMP-2, -4 and -7 on human osteoblasts is required. Methods: Cells of the immortalised human fetal osteoblastic line hFOB 1.19 were exposed to recombinant human (rh) BMP-2, -4 and -7. In addition, primary human osteoblasts were exposed to rhBMP-7. Cell proliferation was measured using a colorimetric assay. Apoptotic cells were detected using the TUNEL assay. Results: The hFOB cells exposed in a dose-dependent manner to rhBMP-2, -4 and -7 had significantly lower rates of proliferation than non-treated cells, (p
    Original languageEnglish
    Pages (from-to)53-60
    JournalThe Journal of Musculoskeletal and Neuronal Interactions
    Volume9
    Issue number1
    Publication statusPublished - 2009

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