This study examined whether the anti-oxidants ascorbic acid, alpha- or gamma-tocopherol, could modify adrenocorticotrophic hormone (ACTH)-hypertension in Sprague-Dawley rats, a model associated with increased oxidative stress. Systolic blood pressure (SBP) was measured by the tail-cuff method. After four days of ascorbic acid (AA) (200mg/kg/day drinking) or a-tocopherol (500 mglkgld i.p. or feed), rats were co-administered ACTH (0.2 mg/kg/day s.c.) or saline for 11 days (prevention studies). In reversal studies, ACTH/saline was administered for 15 days, and from day 9, alpha- or gamma-tocopherol (20 mg/kg/day) was added. ACTH increased SBP compared to saline (p < 0.05). AA or a-tocopherol failed to prevent and alpha- or gamma-tocopherol failed to reverse ACTH-induced hypertension. Thus, neither vitamin C (water soluble) nor E (lipid soluble) modified ACTH-induced hypertension in the rat.
Schyvens, C. G., Andrews, M. C., Tam, T., Mori, T., Croft, K., Mckenzie, K. U. S., Whitworth, J. A., & Zhang, Y. (2007). Antioxidant Vitamins and Adrenocorticotrophic Hormone-Induced Hypertension in Rats. Clinical and Experimental Hypertension, 29(7), 465-478. https://doi.org/10.1080/10641960701615774