TY - JOUR
T1 - Antimicrobial-resistant Bacteroides fragilis in Thailand and their inhibitory effect in vitro on the growth of Clostridioides difficile
AU - Imwattana, Korakrit
AU - Kiratisin, Pattarachai
AU - Riley, Thomas V.
PY - 2022/2
Y1 - 2022/2
N2 - Objectives: The aim of this study was to investigate antimicrobial-resistant Bacteroides fragilis in Thailand and possible effects of such strains on human health and disease. Methods: Phenotypic antimicrobial susceptibility testing was performed on 17 clinical B. fragilis isolates. The genome of one isolate was sequenced and analysed to explore its resistance genotype. An in vitro growth assay was conducted to evaluate the inhibitory effect of B. fragilis on Clostridioides difficile. Results: There was a high prevalence of clindamycin (71%), meropenem (47%) and moxifloxacin (29%) resistance. Most strains remained susceptible to metronidazole, but one had high-level metronidazole resistance conferred by a nimD-containing plasmid. B. fragilis displayed an in vitro inhibitory effect on the growth of C. difficile and a drug-resistant strain retained this inhibition in the presence of clindamycin. Conclusions: Antimicrobial resistance was seen in Thai B. fragils isolates, which may help protect the host against C. difficile infection.
AB - Objectives: The aim of this study was to investigate antimicrobial-resistant Bacteroides fragilis in Thailand and possible effects of such strains on human health and disease. Methods: Phenotypic antimicrobial susceptibility testing was performed on 17 clinical B. fragilis isolates. The genome of one isolate was sequenced and analysed to explore its resistance genotype. An in vitro growth assay was conducted to evaluate the inhibitory effect of B. fragilis on Clostridioides difficile. Results: There was a high prevalence of clindamycin (71%), meropenem (47%) and moxifloxacin (29%) resistance. Most strains remained susceptible to metronidazole, but one had high-level metronidazole resistance conferred by a nimD-containing plasmid. B. fragilis displayed an in vitro inhibitory effect on the growth of C. difficile and a drug-resistant strain retained this inhibition in the presence of clindamycin. Conclusions: Antimicrobial resistance was seen in Thai B. fragils isolates, which may help protect the host against C. difficile infection.
KW - Antimicrobial resistance
KW - Bacteroides fragilis
KW - Clostridioides difficile
UR - http://www.scopus.com/inward/record.url?scp=85122124021&partnerID=8YFLogxK
U2 - 10.1016/j.anaerobe.2021.102505
DO - 10.1016/j.anaerobe.2021.102505
M3 - Article
C2 - 34973432
AN - SCOPUS:85122124021
SN - 1075-9964
VL - 73
JO - Anaerobe
JF - Anaerobe
M1 - 102505
ER -