Antigenic and Genetic Typing of Whataroa Viruses in Australia

S.M. Saleh, M. Poidinger, J.S. Mackenzie, A.K. Broom, Michael Lindsay, R.C. Russell, M.J. Cloonan, C.H. Calisher, G.W. Burgess, R.A. Hall

Research output: Contribution to journalArticlepeer-review

5 Citations (Web of Science)


We recently characterized three novel alphaviruses isolated from mosquitoes captured in New South Wales, Australia. Initial cross-neutralization studies revealed antigenic similarity to the Sindbis virus (SINV)-like Whataroa virus (WHAV), heretofore found only in New Zealand. Nucleotide sequence analysis showed that the WHAV-Iike viruses shared >99% nucleotide sequence similarity with each other, and 96-97% similarity with prototype WHAV. Enzyme-linked immunosorbent assay reactions of a panel of monoclonal antibodies to SINV showed that the novel WHAV-Iike viruses displayed identical binding patterns and were antigenically distinct from all SINV isolates examined. Although these viruses displayed a similar binding pattern to prototype WHAV, three monoclonal antibodies discriminated them from the New Zealand virus. Our results suggest that these novel alphaviruses are antigenic variants of WHAV and represent the first reported isolations of this virus from outside New Zealand. The monoclonal antibodies used in this study will be useful for typing new SINV and SINV-like isolates.
Original languageEnglish
Pages (from-to)262-267
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number3
Publication statusPublished - 2004


Dive into the research topics of 'Antigenic and Genetic Typing of Whataroa Viruses in Australia'. Together they form a unique fingerprint.

Cite this