Anti-Interleukin-10 Unleashes Transcriptional Response to Leishmanial Antigens in Visceral Leishmaniasis Patients

Om Prakash Singh, Genevieve Syn, Susanne Nylén, Christian Engwerda, David Sacks, Mary E. Wilson, Rajiv Kumar, Jaya Chakravarty, Shyam Sundar, Jenefer M. Blackwell, Michaela Fakiola

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Visceral leishmaniasis (VL; Leishmania donovani) cases produce interferon-γ and tumor necrosis factor in response to soluble leishmanial antigen (SLA) in whole-blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL-10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti-IL-10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6, and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL-10 as a potent immune modulator in VL.

Original languageEnglish
Pages (from-to)517-521
Number of pages5
JournalThe Journal of infectious diseases
Volume223
Issue number3
DOIs
Publication statusPublished - 13 Feb 2021

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