Anti-inflammatory effects of interleukin-4 interleukin-10, and transforming growth factor-beta on human placental cells in vitro

VJ Goodwin, TA Sato, MD Mitchell, Jeffrey Keelan

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    37 Citations (Scopus)

    Abstract

    PROBLEM: To determine the effects of anti-inflammatory cytokines on the production of inflammatory mediators by placental cells.METHOD OF STUDY: Cells from term human placentas were isolated and cultured in vitro in the presence of anti-inflammatory cytokines interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-beta. Their effects on the production of IL-8 and prostaglandin E-2 (PGE(2)) were investigated under basal conditions and after stimulation with IL-1 beta and tumor necrosis factor (TNF)-alpha.RESULTS: Both IL-10 and IL-4 inhibited IL-1 beta- and TNF-alpha-induced PGE(2) production but had no significant effects on the production of PGE(2) under basal conditions. TGF-beta(1) was without effect in stimulated cells, whereas under basal conditions TGF-beta(1) stimulated PGE(2) production. Similar trends were seen for IL-8 production, with the exceptions that TGF-beta(1) decreased the TNF-alpha-induced production and IL-4 decreased basal IL-8 production.CONCLUSIONS: The anti-inflammatory effects shown by IL-4, IL-10, and (to lesser extent) TGF-beta may play a role in ameliorating the potentially harmful effects of pro-inflammatory mediators in the feto-placental unit.
    Original languageEnglish
    Pages (from-to)319-325
    JournalAmerican Journal of Reproductive Immunology
    Volume40
    Publication statusPublished - 1998

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