Angiopoietin-1 is required for Schlemm’s canal development in mice and humans

Benjamin R. Thomson, Tomokazu Souma, Stuart W. Tompson, Tuncer Onay, Krishnakumar Kizhatil, Owen M. Siggs, Liang Feng, Kristina N. Whisenhunt, Tammy L. Yanovitch, Luba Kalaydjieva, Dimitar N. Azmanov, Simone Finzi, Christine E. Tanna, Alex W. Hewitt, David A. Mackey, Yasmin S. Bradfield, Emmanuelle Souzeau, Shari Javadiyan, Janey L. Wiggs, Francesca Pasutto & 6 others Xiaorong Liu, Simon W.M. John, Jamie E. Craig, Jing Jin, Terri L. Young, Susan E. Quaggin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm’s canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed by functional analysis of ANGPT1 variants in a combined in silico, in vitro, and in vivo approach, supporting a causative role for ANGPT1 in disease. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.

Original languageEnglish
Pages (from-to)4421-4436
Number of pages16
JournalJournal of Clinical Investigation
Volume127
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Angiopoietin-1
Angiopoietins
Glaucoma
Trabecular Meshwork
Aqueous Humor
Blindness
Intraocular Pressure
Knockout Mice
Computer Simulation
Alleles
Ligands
Mutation

Cite this

Thomson, B. R., Souma, T., Tompson, S. W., Onay, T., Kizhatil, K., Siggs, O. M., ... Quaggin, S. E. (2017). Angiopoietin-1 is required for Schlemm’s canal development in mice and humans. Journal of Clinical Investigation, 127(12), 4421-4436. https://doi.org/10.1172/JCI95545
Thomson, Benjamin R. ; Souma, Tomokazu ; Tompson, Stuart W. ; Onay, Tuncer ; Kizhatil, Krishnakumar ; Siggs, Owen M. ; Feng, Liang ; Whisenhunt, Kristina N. ; Yanovitch, Tammy L. ; Kalaydjieva, Luba ; Azmanov, Dimitar N. ; Finzi, Simone ; Tanna, Christine E. ; Hewitt, Alex W. ; Mackey, David A. ; Bradfield, Yasmin S. ; Souzeau, Emmanuelle ; Javadiyan, Shari ; Wiggs, Janey L. ; Pasutto, Francesca ; Liu, Xiaorong ; John, Simon W.M. ; Craig, Jamie E. ; Jin, Jing ; Young, Terri L. ; Quaggin, Susan E. / Angiopoietin-1 is required for Schlemm’s canal development in mice and humans. In: Journal of Clinical Investigation. 2017 ; Vol. 127, No. 12. pp. 4421-4436.
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abstract = "Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm’s canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed by functional analysis of ANGPT1 variants in a combined in silico, in vitro, and in vivo approach, supporting a causative role for ANGPT1 in disease. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.",
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Thomson, BR, Souma, T, Tompson, SW, Onay, T, Kizhatil, K, Siggs, OM, Feng, L, Whisenhunt, KN, Yanovitch, TL, Kalaydjieva, L, Azmanov, DN, Finzi, S, Tanna, CE, Hewitt, AW, Mackey, DA, Bradfield, YS, Souzeau, E, Javadiyan, S, Wiggs, JL, Pasutto, F, Liu, X, John, SWM, Craig, JE, Jin, J, Young, TL & Quaggin, SE 2017, 'Angiopoietin-1 is required for Schlemm’s canal development in mice and humans' Journal of Clinical Investigation, vol. 127, no. 12, pp. 4421-4436. https://doi.org/10.1172/JCI95545

Angiopoietin-1 is required for Schlemm’s canal development in mice and humans. / Thomson, Benjamin R.; Souma, Tomokazu; Tompson, Stuart W.; Onay, Tuncer; Kizhatil, Krishnakumar; Siggs, Owen M.; Feng, Liang; Whisenhunt, Kristina N.; Yanovitch, Tammy L.; Kalaydjieva, Luba; Azmanov, Dimitar N.; Finzi, Simone; Tanna, Christine E.; Hewitt, Alex W.; Mackey, David A.; Bradfield, Yasmin S.; Souzeau, Emmanuelle; Javadiyan, Shari; Wiggs, Janey L.; Pasutto, Francesca; Liu, Xiaorong; John, Simon W.M.; Craig, Jamie E.; Jin, Jing; Young, Terri L.; Quaggin, Susan E.

In: Journal of Clinical Investigation, Vol. 127, No. 12, 01.12.2017, p. 4421-4436.

Research output: Contribution to journalArticle

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AU - Thomson, Benjamin R.

AU - Souma, Tomokazu

AU - Tompson, Stuart W.

AU - Onay, Tuncer

AU - Kizhatil, Krishnakumar

AU - Siggs, Owen M.

AU - Feng, Liang

AU - Whisenhunt, Kristina N.

AU - Yanovitch, Tammy L.

AU - Kalaydjieva, Luba

AU - Azmanov, Dimitar N.

AU - Finzi, Simone

AU - Tanna, Christine E.

AU - Hewitt, Alex W.

AU - Mackey, David A.

AU - Bradfield, Yasmin S.

AU - Souzeau, Emmanuelle

AU - Javadiyan, Shari

AU - Wiggs, Janey L.

AU - Pasutto, Francesca

AU - Liu, Xiaorong

AU - John, Simon W.M.

AU - Craig, Jamie E.

AU - Jin, Jing

AU - Young, Terri L.

AU - Quaggin, Susan E.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm’s canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed by functional analysis of ANGPT1 variants in a combined in silico, in vitro, and in vivo approach, supporting a causative role for ANGPT1 in disease. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.

AB - Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm’s canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed by functional analysis of ANGPT1 variants in a combined in silico, in vitro, and in vivo approach, supporting a causative role for ANGPT1 in disease. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.

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Thomson BR, Souma T, Tompson SW, Onay T, Kizhatil K, Siggs OM et al. Angiopoietin-1 is required for Schlemm’s canal development in mice and humans. Journal of Clinical Investigation. 2017 Dec 1;127(12):4421-4436. https://doi.org/10.1172/JCI95545