Analyzing biases of nitrogen contents and δ 15N values arising from acidified marine sediments with different CaCO3 concentrations

Yajun Peng, Dongyan Liu, Yujue Wang, Pierre Richard, John K. Keesing

Research output: Contribution to journalArticle

Abstract

In some studies, the researchers pretreated and measured organic carbon, nitrogen and their isotopes (δ13C and δ15N) of marine sediment together, to save costs and resources of analysis. However, the procedure of acidification to remove inorganic carbon for analysing δ13C can affect the values of nitrogen and δ15N, and the biases vary a lot depending on the CaCO3 contents of sediments. In this study, the biases of total nitrogen (TN) and δ15N values arising from acidified sediments were compared between the CaCO3-poor (1%–16%) and CaCO3- rich (20%–40%) samples. TN and δ15N values were altered during acid treatment (without centrifugation) that possibly led to N-containing compounds volatilization. For CaCO3-poor samples, acidification led to a range of 0%–40% TN losses and 0‰–2‰ shift in δ15N values; and 10%–60% TN losses and 1‰–14‰ shift in δ15N values for CaCO3-rich samples. The biases from most samples exceeded the precision of the instrument (0.002% for TN and 0.08‰ for δ15N), and high biases could mislead our judgment for the environmental implication of the data. Thus, avoiding co-analysis of organic carbon, nitrogen and their isotopes (δ13C and δ15N) in sediments, even for CaCO3- poor marine sediments, is necessary.

Original languageEnglish
Pages (from-to)1-5
JournalActa Oceanologica Sinica
Volume37
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

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marine sediments
nitrogen content
marine sediment
Sediments
Nitrogen
nitrogen
Acidification
Organic carbon
sediments
acidification
Isotopes
carbon
isotopes
organic carbon
isotope
sediment
sampling
Centrifugation
inorganic carbon
volatilization

Cite this

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title = "Analyzing biases of nitrogen contents and δ 15N values arising from acidified marine sediments with different CaCO3 concentrations",
abstract = "In some studies, the researchers pretreated and measured organic carbon, nitrogen and their isotopes (δ13C and δ15N) of marine sediment together, to save costs and resources of analysis. However, the procedure of acidification to remove inorganic carbon for analysing δ13C can affect the values of nitrogen and δ15N, and the biases vary a lot depending on the CaCO3 contents of sediments. In this study, the biases of total nitrogen (TN) and δ15N values arising from acidified sediments were compared between the CaCO3-poor (1{\%}–16{\%}) and CaCO3- rich (20{\%}–40{\%}) samples. TN and δ15N values were altered during acid treatment (without centrifugation) that possibly led to N-containing compounds volatilization. For CaCO3-poor samples, acidification led to a range of 0{\%}–40{\%} TN losses and 0‰–2‰ shift in δ15N values; and 10{\%}–60{\%} TN losses and 1‰–14‰ shift in δ15N values for CaCO3-rich samples. The biases from most samples exceeded the precision of the instrument (0.002{\%} for TN and 0.08‰ for δ15N), and high biases could mislead our judgment for the environmental implication of the data. Thus, avoiding co-analysis of organic carbon, nitrogen and their isotopes (δ13C and δ15N) in sediments, even for CaCO3- poor marine sediments, is necessary.",
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Analyzing biases of nitrogen contents and δ 15N values arising from acidified marine sediments with different CaCO3 concentrations. / Peng, Yajun; Liu, Dongyan; Wang, Yujue; Richard, Pierre; Keesing, John K.

In: Acta Oceanologica Sinica, Vol. 37, No. 8, 01.08.2018, p. 1-5.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analyzing biases of nitrogen contents and δ 15N values arising from acidified marine sediments with different CaCO3 concentrations

AU - Peng, Yajun

AU - Liu, Dongyan

AU - Wang, Yujue

AU - Richard, Pierre

AU - Keesing, John K.

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