Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix

J. Ito; J.L. Heazlewood; Harvey Millar

doi:10.1021/pr060403j

Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix

J. Ito, J.L. Heazlewood, Harvey Millar

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51 Citations (Scopus)

Abstract

The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.

Original language	English
Pages (from-to)	3459-3469
Journal	Journal of Proteome Research
Volume	5
Issue number	12
DOIs	https://doi.org/10.1021/pr060403j
Publication status	Published - 2006

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10.1021/pr060403j

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title = "Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix",

abstract = "The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.",

author = "J. Ito and J.L. Heazlewood and Harvey Millar",

year = "2006",

doi = "10.1021/pr060403j",

language = "English",

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journal = "Journal of Proteome Research",

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T1 - Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix

AU - Ito, J.

AU - Heazlewood, J.L.

AU - Millar, Harvey

PY - 2006

Y1 - 2006

N2 - The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.

AB - The interactions of ATP inside plant mitochondria were investigated by identifying the soluble nucleotide binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D IEF-SDS-PAGE and identified by ESI-Q-TOF MS/MS. A range of highly enriched proteins were identified from the mitochondrial proteome, including 14-3-3 proteins and RNA binding proteins, as well as proteins known to contain nucleotide binding domains and/or to be inhibited or stimulated by ATP.

U2 - 10.1021/pr060403j

DO - 10.1021/pr060403j

M3 - Article

SN - 1535-3893

VL - 5

SP - 3459

EP - 3469

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 12

ER -

Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix

Abstract

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