Analysis of the Plant Mitochondrial Transcriptome

Kevin Baudry, Etienne Delannoy, Catherine Colas des Francs-Small

Research output: Chapter in Book/Conference paperChapterpeer-review

Abstract

Transcriptome analyses are widely performed, but the analysis of organelle genome expression is often overlooked. In this chapter, we describe three methods to analyse the accumulation, splicing, editing and processing of plant mitochondrial transcript expression: a classical RT-qPCR assay, an RNA-Seq approach with its bioinformatical and statistical analysis pipeline, as well as a useful complementary technique, the Northern-blot analysis, using short biotinylated oligonucleotides as probes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages235-262
Number of pages28
Volume2363
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2363
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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